重组人白血病抑制因子体外诱导人巨核细胞白血病DAMI细胞株凋亡与P73蛋白表达  

Apoptosis and P73 Expression Induced by Recombinant Human Leukemia Inhibitory in DAMI Cell Line

作　　者：毛彦娜[1] 徐学聚[1] 白松婷[1] 王飞[1] 卢洁[1] 张艳华[1] 

机构地区：[1]郑州大学第一附属医院儿科,郑州450052

出　　处：《实用儿科临床杂志》2007年第15期1156-1157,共2页Journal of Applied Clinical Pediatrics

摘　　要：目的探讨重组人白血病抑制因子(rh-LIF)诱导人巨核细胞白血病DAMI细胞株凋亡和P73蛋白表达,探讨其作用机制。方法应用锥虫蓝拒染法、CCK-8法观察不同质量浓度(1～40μg/L)rh-LIF作用6、12、24、48h后对DAMI细胞株增殖及活力影响,用流式细胞仪测定早期凋亡指标Annexin V,免疫组织化学法检测用药前后P73蛋白表达变化。结果低质量浓度(1、2μg/L)rh-LIF对DAMI细胞增殖无明显抑制,诱导凋亡作用不明显,P73蛋白阳性表达低(P>0.05);当质量浓度>5μg/L时其作用随培养时间延长及药物质量浓度增加而增强(P<0.05)。结论低水平rh-LIF对DAMI细胞增殖无明显抑制,高水平rh-LIF抑制DAMI细胞增殖并诱导其凋亡,用药后P73蛋白阳性表达有明显增高。Objective To explore the apoptosis and P73 protein expression induced by recombinant human leukemia inhibitory（ rh - LIF） in DAMI cell line and explore its cellular and molecular mechanisms, Methods Cell proliferation and viability were analyzed by trypan blue exclusion assay and CCK -8 assay and to delect different concentrations rh - LIF（ 1 -40 μg/L） for 6,12,24,48 h in DAMI cell line. Flow cytometry were perfomed to detect index of apoptosis - Annexin V and the changes of P73 protein expression before and after drugs induction. Resuits The inhibitory rate and apopotosis rate were not significant when rh - LIF concentration was low - dose（ 1,2 μg/L） and P73 protein positive expression was low （P 〉 0. 05）. However, inhibit the DAMI cell line proliferation and induce apoptosis in a dose and time dependent manner when the concentration of rh - LIF was over 5 μg/L（ P 〈 0. 05 ）. Conclusion Low - dose rh - LIF inhibit proliferation of the DAMI cell quietly while large dose rh - LIF suppresses the DAMI cell multiplication and induces apoptosis, after the medication P73 protein positive expression increass obviously.

关 键 词：重组人白血病抑制因子 凋亡 DAMI细胞株 P73蛋白 

分 类 号：R733.7[医药卫生—肿瘤]