非肥胖糖尿病/重症联合免疫缺陷小鼠白血病微血管生成模型的建立  

Construction of Microvessel Angiogenesis Model in Nonobese Diabetic/Severe Combined Immunodeficient Disease Leukemia Mice

作　　者：郭海霞[1] 黎阳[1] 邹燕琴[2] 李文益[1] 

机构地区：[1]中山大学附属第二医院儿科,广州510120 [2]中山大学附属第二医院科研科,广州510120

出　　处：《实用儿科临床杂志》2007年第15期1172-1173,1196,共3页Journal of Applied Clinical Pediatrics

基　　金：广东科技计划项目资助(2006B36301003)

摘　　要：目的尝试建立非肥胖糖尿病/重症联合免疫缺陷(NOD/SCID)小鼠白血病微血管生成模型。方法NOD/SCID小鼠分为A组:空白对照组,B组:注射血管内皮细胞(EC)组,C组:建立白血病模型后注射EC组。通过免疫组织化学对NOD/SCID小鼠骨髓血管生成及全身肿瘤负荷情况进行评价。结果C组骨髓微血管密度明显>A、B组,白血病新生的微血管分支多紊乱,管腔较不规则,血管形态幼稚。结论成功建立NOD/SCID小鼠白血病微血管生成模型,可用于抗白血病血管生成及相关实验研究。Objective To construct microvessel angiogenesis model in nonobese diabetic/severe combined immunodeficient disease（NOD/ SCID） leukemia mice. Methods Divided NOD/SCID mice into group A：blank group;group B：endothelial cell（EC） injection group;group C： leukemia model mice with EC injection group. Microvessel density（MVD） in bone marrow was calculated with cnemis slice after iwanunohistochemistry dyeing. Evaluated leukemia burden in leukemia model mice. Results Bone marrow MVD in group C was significantly more than group B. Microvessels in leukemia mice had disordered branches and irregular cavity. The morphism of vessels was immature. Conclusions Microvessel angingenesis model in NOD/SCID leukemia mice has been constructed successfully. It can be used in the study of anti - angingenesis in leukemia and relative researches.

关 键 词：血管生成 动物模型 白血病 

分 类 号：R-332[医药卫生]