抗氧化剂PDTC对酸性环境诱导的人卵巢癌SKOV3细胞IL-8表达的作用及机制  

Effect of antioxidant PDTC on expression of IL-8 in human ovarian caner cell line SKOV3 in acidic environment

作　　者：王琨[1,2] 崔满华[1] 何凯[3] 

机构地区：[1]吉林大学第二医院妇产科 [2]南方医科大学附属深圳妇幼保健院,广东深圳518028 [3]黑龙江省大庆油田总医院集团龙南医院

出　　处：《吉林大学学报（医学版）》2007年第4期690-694,778,共6页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅基金资助课题(20050410-1)

摘　　要：目的:探讨二硫氨基甲酸吡啶(PDTC)对酸性环境中培养的人卵巢癌细胞SKOV3的增殖活性及白细胞介素8(IL-8)表达的影响,以及细胞核转录因子-κB(NF-κB)在此过程中的作用。方法:将SKOV3细胞分为5组:常规培养液组(对照组)和酸性培养组以及酸性培养液加PDTC组(分别加入终浓度为25、50、100μmol·L-1的PDTC);各组培养12h后,MTT法检测PDTC对SKOV3细胞增殖的影响,ELISA法检测培养上清中IL-8蛋白含量,Western blotting检测核内NF-κB蛋白的表达。结果:SKOV3细胞在酸性环境中IL-8表达水平[(1384.211±42.320)ng·L-1]高于对照组[(617.505±47.850)ng·L-1](P<0.01)。加入不同浓度PDTC(25、50、100μmol·L-1),IL-8表达逐步降低[(1239.053±14.490)、(1113.578±29.140)、(1014.457±55.490)ng·L-1],均低于酸性培养组(P<0.05)。在酸性环境中的SKOV3细胞可观察到颜色较深的核内NF-κB蛋白条带,应用PDTC后NF-κB在核内的蛋白条带颜色变浅。结论:PDTC对酸性环境中SKOV3细胞IL-8的表达具有抑制作用,该作用与PDTC抑制NF-κB的活化有关。Objective To investigate the effect of pyrrolidine dithiocarbamate （PDTC） on the proliferation and expression of IL-8 of SKOV3 cells in acid environment, and the role of nuclear factor κB （NF -κB） in the process. Methods SKOV3 cell lines were divided into 5 groups： normal culture group, acid environment group, acid environment plus PDTC groups （25, 50 and 100 mol·L^-1 PDTC）. All the SKOV3 cells were cultivated for 12 h. The effect of PDTC on the proliferation of SKOV3 in the acid environment was determined by MTT, the content of IL-8 protein in the culture supernatant was detected by ELISA and the expression of NF-κB protein was measured by Western blotting. Results The IL-8 expression level in the acid environment was （1 384. 211±42. 320） ng·L^-1. there was significant difference compared with normal group （617. 505 ng·L^-1 ±47. 850 ng·L^-1 ） （P〈0.01）. The IL-8 expression levels were decreased gradually with the enhancement of PDTC concentration （25, 50, and 100mol·L^-1）,and the values were （1 239.053±14.490）, （1 113.578±29.140） and （1 014.457±55. 490） ng·L^-1, there were significant differences compared with acid environment group （P〈 0.05）. The deeper color protein strap of NF-κB in the SKOV3 cells was found in the acid environment and the color turned pale after SKOV3 cells were treated with PDTC. Conclusion PDTC could suppress the secretion of IL-8 in acid environment by supressing the activation of NF-κB.

关 键 词：卵巢肿瘤 白细胞介素8 NF-ΚB 二硫氨基甲酸吡啶 

分 类 号：R737.31[医药卫生—肿瘤]