膀胱癌病人尿液中的特征物质研究  

Study on the Characteristic Components in the Human Urine with Bladder Cancer

作　　者：朱凤英[1] 虞爱娜[1] 邱意弘[2] 撒应龙[3] 王复[1] 

机构地区：[1]华东理工大学能源系,上海200237 [2]上海交通大学生物医学工程系,上海200240 [3]上海交通大学附属第六人民医院泌尿科,上海200233

出　　处：《分析化学》2007年第8期1132-1136,共5页Chinese Journal of Analytical Chemistry

摘　　要：采用顶空气相色谱/质谱联用法分析膀胱癌病人的尿液,寻找尿液中的挥发性特征物质,以期作为尿液检查诊断膀胱癌的一种方式。选取一组膀胱癌患者,对其尿液进行定性定量分析,并将分析结果与健康人尿液进行比较。进样口温度280℃,进样量100μL;起始柱温45℃,保留3min,以16℃/min的速率程序升温到280℃。结果表明:膀胱癌病人尿液中含有两种特征物质4-庚酮和二甲基硫醚。膀胱癌患者尿液中4-庚酮的浓度范围为0.14～1.32μL/L;二甲基硫醚的浓度范围为0.009～0.73μL/L。推测4-庚酮可能来源于增塑剂邻苯二甲酸二异辛酯(di(2-ethylhexyl)phthalate,DEHP)在人体内的降解;二甲基硫醚来源于含硫化合物在人体内的合成与降解。尿液中4-庚酮和二甲基硫醚的含量与膀胱癌具有相关性。A group of human urines with bladder cancer were qualitatively and quantitatively analyzed under the condition of 280℃ injection temperature, 45℃ initial column temperature, 3 minutes column temperature retaining and 280℃ terminate temperature at the rate of 16℃/min. The analytical results were compared with those of the urine of controls. The characteristic components in the human urine with bladder cancer were 4-heptanone and dimethyl sulfide. The concentrations of 4-heptanone and dimethyl sulfide were ranged from 0. 14 μL/L to 1.32 μL/L and from 0. 009 μL/L to 0.73 μL/L respectively. The results indicate that the 4-heptanone could be caused by the degradation of plasticizer di （2-ethylhexyl） phthalate （DEHP） in the human sulfide. cancer. body The and dimethyl sulfur is caused by the synthesis and degradation of the compounds containing concentration of 4-heptanone and dimethyl sulfide in the urine has a relationship with bladder

关 键 词：膀胱癌 尿液 特征物质 顶空气相色谱/质谱联用 

分 类 号：R737.14[医药卫生—肿瘤]