一种用于化学分析的新方法——荧光毛细分析法  

A New Method for Chemical Analysis: Fluorescence Capillary Analysis

作　　者：李永生[1] 高秀峰[2] 

机构地区：[1]四川大学化学工程学院,四川成都610065 [2]四川大学华西基础医学与法医学院,四川成都610041

出　　处：《光谱学与光谱分析》2007年第8期1565-1569,共5页Spectroscopy and Spectral Analysis

基　　金：教育部留学回国人员科研启动基金项目(2300448);四川大学"214"振兴计划基金项目(0082204127098)资助

摘　　要：提出了一种新方法——荧光毛细分析法(fluorescence capillary analysis,FCA)。FCA法用毛细管做为荧光反应和测定的载体,实现了微量样品的微量分析。本方法特点是操作简便,试样、试剂用量少(<10μL);毛细管及毛细生物反应器可循环使用,测定成本极低,能大大减少环境污染。该研究基于FCA法测定了核黄素、还原型辅酶Ⅰ,基于固定化酶FCA法测定了酒中乙醇,基于固定化核苷酸探针基因FCA法测定了靶核苷酸,都初步得到了满意的结果。FCA法将可以应用到临床医学、药学、生化、染料工业、发酵食品、环境检测等各个领域中。A new analytical approach called fluorescence capillary analysis （FCA） has been proposed. FCA method uses a capillary as a carrier for fluorescence reaction and determination, and with this method micro analysis of micro samples has been realized. Its advantages are simple to manipulation, thimbleful of sample and reagent volumes （〈10 μL）, repeated use of capillary, and the lowest test cost. FCA method can consumedly decrease the pollution of the environment. Based on FCA and an immobilization enzyme FCA, VB2, NADH, and ethanol in alcohol drinks have been determined. Based on an immobilization probe gene FCA, target nucleotide also has been determined. These results were satisfactory. FCA method can be used for fermentable food, medicine, biochemistry, industry, etc.

关 键 词：荧光毛细分析法 核黄素 NADH 固定化酶 乙醇 基因 

分 类 号：O657.3[理学—分析化学]