Apelin-13对离体缺血/再灌注大鼠心脏损伤的保护性影响  

Protective effects of apelin-13 on ischemia/reperfusion injury of rat heart

作　　者：曾翔俊[1] 王红霞[1] 芦玲巧[1] 王晓燕[1] 马利权[1] 张立克[1] 唐朝枢[2] 

机构地区：[1]首都医科大学病理生理学教研室,北京100069 [2]北京大学第一医院心血管研究所,北京100034

出　　处：《基础医学与临床》2007年第8期864-867,共4页Basic and Clinical Medicine

基　　金：北京市自然科学基金(7062007);北京市教育委员会科技发展项目(KM200510025009)

摘　　要：目的观察apelin-13对离体大鼠缺血/再灌注心脏损伤的影响,并初步探讨其对apelin受体APJ(putative re-ceptor protein related to the angiotensin receptorAT1)及细胞信号Akt1/2的影响。方法Langendorff装置恒流灌注大鼠离体心脏,采用停灌/复灌方式复制缺血/再灌注模型;观察缺血/再灌注期间心脏收缩期左心室内压上升的最大变化速率(+LVdp/dtmax)及舒张期左心室内压下降的最大变化速率(-LVdp/dtmax);RT-PCR和Western blot测定组织中APJ受体mRNA和Akt1/2蛋白的表达情况。结果Apelin-13可以增加缺血/再灌注损伤心脏的±dp/dtmax(P<0·01);可以明显增强心肌组织中Akt1/2的表达;缺血/再灌注可以引起心肌组织中APJ受体表达上调。结论Apelin-13拮抗缺血/再灌注引起的心脏收缩及舒张功能障碍;可能与组织中APJ受体表达上调引起Akt1/2表达增强有关。Objective To observe the protective effects of apelin-13 on on the expression of APJ （putative receptor protein related to the angiotensin receptor AT1 ） and Akt1/2 in ischemia/reperfusion injury rat heart. Methods Langendorff device was used to perfuse isolated rat heart with constant flow. The ischemia/reperfusion models of rat heart were made by stopping and reperfusion KH fluid to the heart; observing the change of maximal rate of the pressure increase（ ＋ dp/dmax） and maximal rate of the pressure decrease（-dp/dmax） of left ventricle in rat heart. RT-PCR was used to assay the expression of APJ mRNA in rat heart, Western blot was used to assay Akt1/2 protein in rat heart. Results The groups given apelin-13 had higher ＋ LVdp/dmax（P 〈0. 01 ） ; and resulted in hyperexpression of Akt1/2 protein （ P 〈 0. 05 ）, while the expression of APJ mRNA was up regulated in IR group （P 〈 0. 05）. Conclusion Apelin-13 can resist the functional impairment in rat heart caused by ischemia/reperfusion injury, and the protecting effects of apelin-13 may be explained by in up regulated expression of APJ in rat heart, and the APJ may up regulate the expression of Akt1/2.

关 键 词：缺血/再灌注损伤 apelin多肽 心肌 

分 类 号：R363[医药卫生—病理学]