癌胚抗原阴性的结直肠癌检测和结直肠癌预后相关生物标记  

Detection of CEA negative colorectal cancer and prognostic biomarkers of colorectal cancer

作　　者：屠世良[1] 颜怀军[2] 郦卫星[1] 李永哲[3] 陈盈[1] 李宁 许洋 

机构地区：[1]浙江省人民医院,浙江杭州310014 [2]空军杭州疗养院,浙江杭州310007 [3]中国医学科学院中国协和医科大学北京协和医院,北京100730 [4]吴阶平医学基金会,北京100062

出　　处：《基础医学与临床》2007年第8期926-931,共6页Basic and Clinical Medicine

摘　　要：目的探讨结直肠癌的发生、发展过程中患者血清质谱多肽蛋白图谱的变化,筛选与结直肠癌预后及癌胚抗原(CEA)阴性检测相关的肿瘤标记分子。方法用蛋白指纹图谱技术检测结直肠癌,结直肠管状腺瘤患者和健康者血清质谱多肽蛋白图谱。结果初步筛选出对结直肠癌有代表性的7个差异蛋白;2个与其淋巴结转移相关的差异蛋白;4个与其远处转移相关的差异蛋白;3个在其根治性切除后表达下降的差异蛋白;而由3398·3u、5477·1u和8453·9u组成的诊断模型对CEA阴性表达结直肠癌的阳性检测率为100%。结论蛋白指纹图谱技术可筛选出有意义的生物标记差异蛋白,这对结直肠癌预后判断、CEA阴性的结直肠癌检测和改变结直肠癌的进程具有重要意义。Objective To study serum proteomic fingerprints of colorectal cancer during onset and progression and to screen tumor markers related to prognosis. Methods Serum from colorectal cancer patients, non-cancer patients, and healthy control were profiled using WCX ProteinChip or magnetic beads and analyzed by mass spectrometry. Results Seven protein peaks were found related to colorectal cancer. Several peaks were closely related to lymph node metastasis, distal organ metastasis and decreased after surgery. The diagnostic model composed of 3398.3, 5477. 1,8453.9 u can detect CEA negative colorectal cancer in 100%. Conclusion Protein fingerprinting technology （PFT） in conjunction with bioinformatics can significantly identify novel biomarkers in the serum of colorectal cancer patients with potential values for prognostic evaluation, detection of CEA negative colorectal cancer and changing its progression.

关 键 词：结直肠癌 蛋白指纹图谱技术 质谱 

分 类 号：R735.3[医药卫生—肿瘤]