脑出血大鼠神经元线粒体内细胞色素C释放及中药方剂的干预效应  

Effects of various traditional Chinese therapies on the release of cytochrome C from neuronal mitochondria after intracerebral hemorrhage in rats

作　　者：梁清华[1] 谢映[1] 包太成[1] 熊新贵[1] 陈疆[1] 刘晓春[1] 曾光[1] 

机构地区：[1]中南大学湘雅医院中西医结合研究所,湖南省长沙市410008

出　　处：《中国组织工程研究与临床康复》2007年第34期6789-6792,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：湖南省自然科学基金(01JJY2024);湖南省科技基金(1013-50)~~

摘　　要：目的:观察大鼠脑出血后血肿周围神经元线粒体内细胞色素C释放规律和血肿变化情况,探讨不同中医治法的干预作用。方法:实验于2003-10/2004-03在中南大学湘雅医院中西医结合研究所省级重点实验室完成。①实验分组:125只SD大鼠分为正常组5只,假手术组20只,模型组20只和大承气汤组20只、涤痰汤组20只、平肝熄风汤组20只、血府逐瘀汤组20只。假手术组,模型组,中药治疗组又分为6h,1,3,5d4个观察时间点,每个时间点5只。②实验干预:用Ⅶ胶原酶脑内立体定位注射诱导大鼠脑出血模型。假手术组以同样方法注入灭菌生理盐水。正常组大鼠自由进食饮水,假手术组蒸馏水2mL灌胃,2次/d,各治疗组造模后即灌服大承气汤、涤痰汤、平肝熄风汤、血府逐瘀汤,用药量按体表面积计算为70kg成人的3倍,每次2mL,2次/d灌胃。共4周。正常组大鼠最后一次进食后6h时处死。其余各组于最后一次给药后6h,1d,3d,5d处死观察各项指标。③实验评估:免疫组织化学法检测神经元线粒体内细胞色素C的释放情况,脑组织冠状切开测量不同时间点血肿直径。结果:125只大鼠均进入结果分析。①脑组织细胞色素C阳性细胞计数:正常组未见阳性细胞;假手术组仅于6h,1d时见少数阳性细胞;模型组和各中药治疗组6h即有阳性细胞表达,1d时阳性细胞达到高峰;3d阳性细胞数减少;5d阳性细胞继续减少。1,3,5d时各中药治疗组低于模型组,差异有显著性(P<0.01)。大承气汤组,涤痰汤组高于平肝熄风组(P<0.01)。②血肿体积变化情况:正常组,假手术组无明显血肿形成;模型组和中药治疗组6h即见明显的血肿形成,1d时血肿直径最大,3d时血肿直径变小,5d后血肿直径继续变小。1,3,5d平肝熄风汤组,血府逐瘀汤组小于模型组(P<0.05或<0.01),3,5d时涤痰汤组小于模型组(P<0.01),5d时大承气汤组小于模型组(P<0.01);3,5d时平肝熄风汤小于大承气AIM： To observe the effects of various traditional Chinese therapies on the release of cytochrome C （Cyt-C） from neuronal mitochondria around the hematoma after intracerebral hemorrhage in rats and the changes of hematoma. METHODS： The experiment was carried out in the Institute of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University from October 2003 to March 2004. ①125 SD rats were divided into normal group （n =5）, sham operation group （n =20）, model group （n =20） and four Chinese medicine groups including Dachengqi Tang （DCQT） group, Ditan Tang （Dl-r） group, Pinggan Xifeng Tang （PGXFT） group, and Xuefu Zhuyu Tang （XFZYT） group with 20 animals in each group. Additionally, the sham operation group, model group and Chinese medicine groups were subdivided into four time points groups of 6 hours, 1, 3, and 5 days with 5 rats in each subgroup. ②Ⅶ type collagenase was intracerebrally injected to the rats to induce models of intracerebral hemorrhage; the sham operation group was injected with sterile normal saline by the above-mentioned method. The rats of the normal group freely drank and took food. The sham operation group was infused with 2 mL distilled water, twice daily, and the medicine groups were given the four kinds of Chinese medicine immediately after modeling with a dose of three times of 70 kg adults, 2 mL every time, twice daily for 4 weeks. The rats of the normal group were executed 6 hours after the last time foodtaken, while other groups were executed 6 hours, 1, 3 and 5 days after the last administration. ③Immunohistochemical method was used to exam the release of Cyt-C from neuronal mitochondria, and the diameters of hematoma at different time points were measured. RESULTS： 125 rats were all involved in the result analysis. ①No positive cell was found in the normal group, and only a few positive cells appeared in the sham operation group at 6 hours and 1 day; the positive cells were observed in the model group

关 键 词：脑出血 中医治法 细胞色素C 大鼠 

分 类 号：R741[医药卫生—神经病学与精神病学]