遗传性非息肉病性大肠癌组织E-cad，β-cat和MMP-7表达在侵袭转移中的作用  

Expression of E-cadherin,β-catenin,matrix metalloproteinase-7 and its correlation to invasion/metastasis of hereditary nonpolyposis colorectal cancer

作　　者：顾国利[1] 魏学明[1] 王石林[1] 任力[2] 周晓武[1] 李德昌[2] 胡益云[2] 

机构地区：[1]中国人民解放军空军总医院普通外科,北京市100036 [2]中国人民解放军空军总医院病理科,北京市100036

出　　处：《世界华人消化杂志》2007年第18期2031-2036,共6页World Chinese Journal of Digestology

摘　　要：目的:探讨上皮钙黏附蛋白(E-cad)、β-连接素(β-cat)和基质金属蛋白酶-7(MMP-7)在遗传性非息肉病性大肠癌(HNPCC)和散发性大肠癌(sporadic CRC)的表达及与遗传性非息肉病性大肠癌(HNPCC)侵袭转移间的关系.方法:应用免疫组化SP法检测HNPCC(n= 30)、散发性大肠癌(sporadic CRC)(n=30)和正常大肠黏膜(n=8)中E-cad,β-cat和MMP-7的表达情况.结果:E-cad和胞膜表达β-cat在HNPCC中的阳性表达率高于其在sporadic CRC中的表达率(E-cad:86.7% vs 60.0%:胞膜β-cat:83-3% vs 50.0%,均P<0.05).而胞质表达β-cat和MMP-7则相反(胞质β-cat:46.7% vs 80.0%;MMP-7:46.7% vs 86.7%;均P>0.05).两组中E-cad,胞膜表达β-cat,胞质表达β-cat,MMP-7的阳性表达率与患者的性别和肿瘤的大小、部位及分化类型均无关:而与肿瘤的侵犯深度和转移与否密切相关(r=0.732、P<0.05).E-cad与胞膜表达β-cat呈正相关(r=0.477,P<0.05),胞膜表达β-cat与胞质表达β-cat呈负相关(r=-0.419,P<0.05),而胞质表达β-cat与MMP-7呈正相关(r=0.380,P<0.05).结论:E-cad,β-cat和MMP-7在HNPCC和sporadicc CRC中的阳性表达率差异显著.可能是HNPCC在获得诊断时侵袭弱、转移少和预后较好的原因之一.AIM： To detect expression of E-cadherin （E-cad）, β-catenin （β-cat） and matrix metalloproteinase-7 （MMP-7） in hereditary non-polyposis colorectal cancer （HNPCC） and sporadic colorectal cancer （CRC）, and to analyze its relationship to the biological behavior of HNPCC. METHODS： Protein expression of E-cad,β-cat and MMP-7 in 30 specimens of HNPCC, 30 of sporadic CRC and eight of normal colorectal tissues was detected by SP immunohistochemistry. Their corresponding clinical data were analyzed retrospectively. RESULTS： The positive expression rates of E-cad and membrane J3-cat in HNPCC patients were higher than those in sporadic CRC （E-cad, 86.7% vs 60.0%; membrane β-cat, 83.3% vs 50.0%, all P 〈 0.05）. The positive expression rates of β cytoplasm β-cat and MMP-7 in HNPCC patients were lower than those in sporadic CRC patients （cytoplasm β-cat, 46.7% vs 80.0%; MMP-7, 46.7% vs 86.7%; both P 〉 0.05）. Positive rates of E-cad, β-cat and MMP-7 in the two groups were significantly related to depth of invasion and lymph node metastasis （r = 0.732, P 〈 0.05）, but not to sex or the size or position of the tumor. Protein expression of E-cad was positively correlated with expression of membrane β-cat （r = 0.477, P 〈 0.05）, however, expression of membrane β-cat was negatively correlated with expression of cytoplasmic β-cat （r=-0.419, P 〈 0.05）, while expression of cytoplasm β-cat was positively correlated with expression of MMP-7 （r = 0.380, P 〈 0.05）. CONCLUSION： There are remarkable differences in expressions of E-cad, β-cat and MMP-7 between HNPCC and sporadic CRC. This could be the reason that HNPCC tumors are less invasive and have less metastatic potential compared to sporadic cancers.

关 键 词：遗传性非息肉病性大肠癌 散发性大肠癌 上皮钙黏附蛋白 Β-连接素 基质金属蛋白酶-7 病理 免疫组化 

分 类 号：R735.35[医药卫生—肿瘤]