Microwave-assisted Micellar Extraction and Determination of Glycyrrhizic Acid and Liquiritin in Licorice Root by HPLC  

作　　者：孙晨 谢渝春 刘会洲 

机构地区：[1]Chemistry and Cllemical Engineering＇Department, Graduate University of Chinese Academy of Sciences, Beijing.100049, China [2]Laboratory of Separation Science and Engineering, State Key Laboratory of Biochemical Engineenng, lnsUtute otProcess Engineering, Chinese Academy of Sciences, Beijing 100080, China

出　　处：《Chinese Journal of Chemical Engineering》2007年第4期474-477,共4页中国化学工程学报（英文版）

基　　金：Supported by the National Science Fund for Creative Research Groups （No.20221603）, the National Natural Science Foundation of China Key Project （No.20490200） and PetroChina.

摘　　要：The feasibility of emploving non-ionic surfactant （Triton X-100） as an alternative and effective solventfor the microwave-assisted extraction of glycyrrhizic acid （GA） and liquiritin （LQ） from licorice root was studied.The optimal extraction parameters based on the microwave-assisted micellar extraction technique were determined.Under the optimal conditions, i.e. 5% （by volume） Triton X-100, microwave-assisted extraction for 3--5min at 373K, the percentage extraction of active ingredients reached the highest value. The preconcentration tactor for GA and L＇Q （about 13.5） and the extraction efficiency for these two ingredients approached 100% showed the coupling of microwave-assisted extraction and cloud-point extraction could be employed as a new and. effective techniquefor the rapid extraction and preconcentration of pharrnacologically active ingredients from medicinal plants SUCh aslicorice root without disturbing chromatographic analysis.The feasibility of employing non-ionic surfactant(Triton X-100)as an alternative and effective solventfor the microwave-assisted extraction of glycyrrhizic acid(GA)and liquiritin(LQ)from licorice root was studied.The optimal extraction parameters based on the microwave-assisted micellar extraction technique were determined.Under the optimal conditions,i.e.5%(by volume)Triton X-100,microwave-assisted extraction for 3—5min at373K,the percentage extraction of active ingredients reached the highest value.The preconcentration factor for GAand LQ(about 13.5)and the extraction efficiency for these two ingredients approached 100%showed the couplingof microwave-assisted extraction and cloud-point extraction could be employed as a new and effective techniquefor the rapid extraction and preconcentration of pharmacologically active ingredients from medicinal plants such aslicorice root without disturbing chromatographic analysis.

关 键 词：microwave-assisted micellarextraction cloud-point extraction Triton X-100 glycyrrhizic acid LIQUIRITIN 

分 类 号：TQ420.66[化学工程]