利用Cyt b基因由未知鲸骨鉴定出大村鲸  

Identification of Balaenoptera omurai from an unknown whale vertebra based on Cyt b gene sequence

作　　者：马牧[1] 祝茜[1] 李响[1] 孙玉苗[1] 刘莹莹[1] 刘亚楠[1] 陶翠花[1] 

机构地区：[1]山东大学威海分校海洋学院,威海264209

出　　处：《兽类学报》2007年第3期288-292,共5页Acta Theriologica Sinica

基　　金：国家自然科学基金资助项目(40376042);中华人民共和国农业部资助项目<中国海域海洋哺乳动物的研究>;山东大学资助项目<山东沿海鲸豚的物种多样性与保护>

摘　　要：In order to identify an unknown whale vertebra’s species,genomic DNA is extracted from the bone powder by proteinase K and phenol/chloroform,and a partial Cyt b gene sequence as long as 565 bp is amplified by PCR(Polymerase Chain Reaction).The blasting result in GenBank shows that the vertebra sample has maximal similarity with Balaenoptera omurai,it is 99%.It indicates that the vertebra is Balaenoptera omurai.The phylogenetic tree based on UPGMA is consistent with the blasting result.Analysis of software MEGA shows that the Cyt b gene sequence of the vertebra sample has 1-2 nucleotides which are different from the homologous sequence of B.omurai(accession AB201257,AB201256),thus,the sequence is a new haplotype.This is the first record of B.omurai which distributes in the South China Sea.In order to identify an unknown whale vertebra＇s species,genomic DNA is extracted from the bone powder by proteinase K and phenol/chloroform,and a partial Cyt b gene sequence as long as 565 bp is amplified by PCR（Polymerase Chain Reaction）.The blasting result in GenBank shows that the vertebra sample has maximal similarity with Balaenoptera omurai,it is 99%.It indicates that the vertebra is Balaenoptera omurai.The phylogenetic tree based on UPGMA is consistent with the blasting result.Analysis of software MEGA shows that the Cyt b gene sequence of the vertebra sample has 1-2 nucleotides which are different from the homologous sequence of B.omurai（accession AB201257,AB201256）,thus,the sequence is a new haplotype.This is the first record of B.omurai which distributes in the South China Sea.

关 键 词：鲸类脊椎骨 细胞色素B基因 分子进化树 种类鉴定 大村鲸 

分 类 号：Q754[生物学—分子生物学]