三羟异黄酮对血管性痴呆大鼠学习记忆及脑内氧化损伤的影响  

Effect of genistein on learning and memory and oxidative damage in vascular dementia rats

作　　者：李俊垚[1] 于辛[2] 孙宏[2] 

机构地区：[1]山东省青岛市市立医院急诊内科,266011 [2]第四军医大学2005级博士队,西安市710032

出　　处：《实用医学杂志》2007年第18期2835-2837,共3页The Journal of Practical Medicine

摘　　要：目的:研究植物雌激素三羟异黄酮(GST)对血管性痴呆模型大鼠学习记忆的影响及其作用机制。方法:采用双侧颈总动脉结扎再灌注合并硝普钠降压法制作大鼠血管性痴呆模型,所有大鼠被分为模型对照组(O组)、假手术对照组(C组)、三羟异黄酮组(GST组)和苯甲雌二醇组(BE2组)4组,采用大鼠跳台法、Morris水迷宫法检测各组大鼠学习记忆能力,并测定大鼠脑组织中超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧物酶(GSHPx)的含量。结果:GST组与O组相比,GST明显提高模型大鼠学习记忆能力,降低MDA,提高SOD、GSHPx的含量。结论:GST可能通过抗氧化损伤、清除自由基的作用,从而改善模型大鼠的学习记忆能力。Objective To observe the effect of genistein on learning and memory and oxidative damage in vascular dementia rats. Methods A rat model of vascular dementia was constructed by ligating bilateral common carotid arteries, then reperfusing, and administrating nitroprusside sodium. The rats were divided into four groups： control group, sham-operated group, genistein group （GST group）, and estradiol benzoate group （BE2 group）. Morris water maze and stand jumping were performed to measure learning and memory in the rats. The contents of superoxide dismutase （SOD）, malonaldehyde （MDA）, glutathione peroxidase （GSHPx） were detected in the cerebral tissue. Results As compared with the control group, genistein significantly improved the ability of learning and memory, decreased MDA levels, and increased the contents of SOD and GSHPx. Conclusion Genistein can significantly improve the ability of learning and memory in vascular dementia rats by inhibiting oxidative damage and eliminating oxygen free radicals.

关 键 词：痴呆 血管性 氧化损伤 三羟异黄酮 超氧化物歧化酶 丙二醛 谷胱甘肽过氧物酶 氧自由基 

分 类 号：R749.1[医药卫生—神经病学与精神病学]