接触室内煤烟污染的肺癌患者血清中细胞生长调控蛋白的研究  

Studies on Serum Regulatory Protein for Cell Growth in Patients with Lung Cancer Exposed to Indoor Coal Burning

作　　者：李健学[1] 李学明[1] 全笑江 邢辉[1] 刘鸿君[1] 方清明[1] 徐坤[1] 

机构地区：[1]中国预防医学科学院环境卫生和卫生工程研究所

出　　处：《中华预防医学杂志》1997年第2期92-94,共3页Chinese Journal of Preventive Medicine

基　　金：国家自然科学基金

摘　　要：为了研究接触室内煤烟污染的肺癌患者的发病机制，我们采用酶连免疫斑点法检测了接触室内煤烟的１９例肺癌患者（Ａ组）、２２例未患肺癌者（Ｂ组）和１９例不接触煤烟的未患肺癌者（Ｃ组）血清中７种细胞生长调控基因编码的蛋白。结果表明，Ａ组ｒａｓ、ｐ５３和ｎｅｕ蛋白含量明显高于Ｂ、Ｃ组，差异有显著性（Ｐ＜０．０５）或极显著性（Ｐ＜０．０１）；Ａ组的阳性样品数为ｒａｓ蛋白６例（其中２例为强阳性，ｐ５３、ｎｅｕ和ｊｕｎ蛋白各３例，ｐ１６蛋白１例；Ｂ组的阳性样品数为ｒａｓ蛋白２例，ｐ１６蛋白１例。提示血清中细胞生长调控蛋白可作为环境相关肺癌的生物标志。Seven kinds of serum regulatory gene coding protein for cell growth were determined with enzyme linked immunodotting in 19 indoor coal burning exposed patients with lung cancer (Group A), 22 exposed cases without lung cancer (Group B), and 19 nonexposed cases without lung cancer (Group C) to study pathogenesis of lung cancer patients exposed to indoor coal burning. Results showed that serum concentrations of ras, p53 and neu protein in Group A were significantly higher than those in Groups B and C, with a statistically significant difference ( P <0.05) or a very significant difference ( P <0.01). Six cases in Group A were positive for ras protein (with two strong positive), three for p53, neu and jun each, and one for p16. Two in Group B were positive for ras protein and one for p16. It suggests that serum regulatory protein for cell growth can be regarded as biological markers for environment related lung cancer.

关 键 词：空气污染 肺肿瘤 细胞生长 调控基因 编码蛋白 

分 类 号：R734.202[医药卫生—肿瘤]