硒酸酯多糖对人乳腺癌细胞株(BCaP-37)生物学作用的研究  

Studies on Biological Effects of Kappa Selenocarrageenan on Human Breast Cancer Cell Line BCaP 37

作　　者：何更生[1,2] 程琮[1,2] 陆瑞芳[1,2] 沈新南[1,2] 吴岷[1,2] 

机构地区：[1]上海医科大学营养与食品卫生学教研室 [2]山东泰山医学院预防医学教研室

出　　处：《中华预防医学杂志》1997年第2期103-106,共4页Chinese Journal of Preventive Medicine

基　　金：1991~1993年卫生部科研基金

摘　　要：为探讨硒酸酯多糖的抗肿瘤机理，用细胞培养技术、图像细胞分析技术（ＩＣＭ）和ＮｏｒｔｈｅｒｎＢｌｏｔ方法检测，经硒酸酯多糖作用后中国人乳腺癌细胞株（ＢＣａＰ－３７）的增殖、细胞周期、总ＤＮＡ含量、细胞核面积以及表皮生长因子受体（ＥＧＦＲ）、癌基因Ｃ－ｅｒｂＢ２ｍＲＮＡ的表达水平。结果发现，硒酸酯多糖（３．０～１２０ｍｇ／Ｌ）对ＢＣａＰ－３７增殖有抑制作用，且有时间和剂量依赖效应。经硒酸酯多糖（１５ｍｇ／Ｌ、６０ｍｇ／Ｌ）处理４天的细胞，在图像细胞分析反映细胞增殖能力的指标中，细胞核面积均显著低于对照组。６０ｍｇ／Ｌ硒酸酯多糖处理的细胞其ＥＧＦＲ、Ｃ－ｅｒｂＢ２ｍＲＮＡ表达水平受到明显抑制。提示硒酸酯多糖能抑制乳腺癌细胞增殖，其机理可能是对ＥＧＦＲ，Ｃ－ｅｒｂＢ２ｍＲＮＡ表达水平的调节。Proliferation, cell cycle, total amount of DNA, area of cell nucleus, as well as epidermal growth factor receptors (EGFR) and expression of oncogene C erbB2 mRNA of Chinese breast cancer cell line (BCaP 37) after being treated with kappa selenocarrageenan were determined by cell culture technique, image cytometry (ICM) and northern blot to explore its anti tumor mechanism. Results revealed 3.0～120 mg/L selenocarrageenan could inhibit proliferation of BCaP 37, with a response of time and dose dependence. The areas of nuclei were significantly lower with ICM in cells treated with 15 or 60 mg/L selenocarrageenan for four days than those in controls ( P <0.01). Levels of EGFR and expression of C erbB2 mRNA were significantly inhibited in cells treated with 60 mg/L selenocarrageenan. It suggests that selenocarrageenan can inhibit proliferation of breast cancer cells through regulation of the levels of EGFR and expresssion of C erbB2 mRNA.

关 键 词：硒酸酯 细胞学技术 乳腺肿瘤 

分 类 号：R737.905[医药卫生—肿瘤]