EphB4/EphrinB2在人脑星形细胞瘤中的表达及其病理学意义  

Expression of EphB4/EphrinB2 in human brain astrocytomas and its pathologic significance

作　　者：古金海[1] 张建中[1] 

机构地区：[1]江西省人民医院神经外科,南昌330006

出　　处：《肿瘤》2007年第8期651-654,共4页Tumor

摘　　要：目的:探讨EphrinB2与其受体EphB4、微血管密度(microvessel density,MVD)和Ki-67在人脑星形细胞瘤中的表达及其病理学意义。方法:采用组织芯片技术和MaxvisionTM快捷免疫组织化学染色方法检测84例人脑星形细胞瘤和12例外伤脑组织中EphB4/EphrinB2、MVD和Ki-67的表达。结果:EphB4/EphrinB2、MVD、和Ki-67标记指数(Ki-67 labelling index,Ki-67 LI)在人脑星形细胞瘤组和对照组之间的差异有统计学意义(P<0.05)。EphB4、EphrinB2、MVD和Ki-67 LI与肿瘤级别呈正相关,MVD和Ki-67 LI与EphB4或EphrinB2蛋白表达正相关,有统计学意义(P<0.05)。结论:EphB4和EphrinB2与人脑星形细胞瘤的分化程度和肿瘤血管生成密切相关,EphB4和EphrinB2的过度表达对肿瘤发展和促进肿瘤血管的生成起重要作用。利用EphB4/EphrinB2、MVD和Ki-67来判断人脑星形细胞瘤的恶性程度和病理特征,是一个较好的参考指标。Objective：To investigate the expressions of EphrinB2 and its receptor EphB4, microvessel density （MVD）, and Ki-67 in human brain astrocytomas and explore their pathological significance. Methods： Expressions of EphB4/EphrinB2, MVD and Ki-67 were detected by tissue microarray and Maxvision shortcut immunohistochemical staining in 84 cases of human astrocytomas and 12 cases of traumatic brain tissues. Results： The difference was significant in expressions of EphB4/EphrinB2, MVD and Ki-67 labeling index （LI） between human astrocytomas and controlled human traumatic brain tissues （P 〈0.05 ）. Expression of EphB4/EphrinB2, MVD and Ki-67 LI had positive correlation with tumor grade. Ki-67 LI and MVD positively correlated with expressions of EphB4 or EphrinB2 protein （P 〈 0.05 ）. Conelusion：There is a close correlation between expression of EphB4/EphrinB2 and differentiation degree, tumor angiogenesis in human brain astrocytoma. The over-expressions of EphB4 and EphrinB2 play an important role in promoting tumor develop- ment and angiogenesis. EphB4/EphrinB2, MVD, and Ki-67 are fine reference parameters to estimate malignancy degree and pathologic features of human brain astrocytomas.

关 键 词：星形细胞瘤 血管内皮生长因子类 蛋白质酪氨酸激酶 

分 类 号：R739.41[医药卫生—肿瘤]