纳米材料根管封闭剂抗微生物作用的实验研究  

In vitro evaluation of the antimicrobial activity of nanomaterial

作　　者：刘志东[1] 任吉芳[1] 

机构地区：[1]长治医学院附属和济医院口腔科,山西长治046000

出　　处：《牙体牙髓牙周病学杂志》2007年第8期458-462,共5页Chinese Journal of Conservative Dentistry

基　　金：山西省农社处资助项目(021076)

摘　　要：目的:观察纳米材料的抗微生物作用。方法:用琼脂扩散法观察4种材料:①氧化锌丁香油糊剂组(ZOE);②羟磷灰石糊剂组(HA);③纳米氧化锌糊剂组(N-ZO);④纳米羟磷灰石糊剂组(NHA)。检测调制后4个时间点(即刻、24h、48h、7d)的抗微生物作用。选择金黄色葡萄球菌、白色念珠菌、粪肠球菌作为实验菌种。结果:4个时间点,HA组和NHA组均没有显示出抑菌作用,ZOE组与N-ZO组的抑菌区域有显著差异(F=315.34,P<0.0001),ZOE组产生的抑菌区域大于N-ZO组;ZOE组与N-ZO组对3种细菌产生的抑菌区域也有显著性差异(F=642.91,P<0.0001),但两组均对粪肠球菌产生的抑菌区域最小;ZOE组与N-ZO组在不同时间点产生的抑菌区域有显著性差异(F=335.31,P<0.0001),但抑菌作用均随时间的延长呈递减趋势。结论:纳米氧化锌糊剂具有较强的抗微生物作用,羟磷灰石糊剂和纳米羟磷灰石糊剂无抑菌作用。AIM： To observe the antimicrobial activity of two kinds of nanomaterial . METHODS： Experimental materials were derided into four groups. Group 1 ： Zinc oxide - eugenol sealer （ ZOE ） （ the control group） ; group 2 ： hydroxyapatite sealer （ HA ） ; group $ ： nano - zinc - oxide sealer （ N - ZO ） ; group 4 ： nanohydroxyapatite sealer （NHA）, The sealers were tested immediately, 24h, 48h and 7 days after manipulation with agar diffusion test respectively. The sealers were placed on the Agar plates after Staphylococcus aureus, Candida albicans and Enterococcusfaecalis had been inoculated. The plates were kept in a 37℃ incubator for 24 h. Zones of inhibition of microbial growth were observed. The antimicrobial activity of the four kinds of materials were assessed according to the zones of inhibition of microbial growth. RESULTS： At four times after manipulation, HA and NHA showed no antimicrobial activity. There were statistical difference among the zones of inhibition of microbial growth of ZOE and N - ZO. ZOE demonstrated higher antimicrobial activity than N - ZO. There were statistical difference among three kinds of bacteria （p 〈0.001 ）. The zones of inhibition of microbial growth of ZOE and N - ZO against Enterococcus faecalis were the smallest. There were statistical difference among four times （P 〈 0.0001 ）. The antimicrobial activity of the sealers reduced with time. （P 〈 0. 0001 ）. CONCLUSION： Nano -zinc -oxide has higher antimicrobial activity. Hydroxypatite and nanohydroxypatite have no antimicrobial activity.

关 键 词：纳米材料 抗微生物作用 琼脂扩散法 

分 类 号：R781.33[医药卫生—口腔医学]