兔VX2肺癌模型的建立和螺旋CT评价  

Establishment of VX2 lung cancer model in rabbit and spiral CT evaluation

作　　者：刘德森[1] 倪才方[2] 秦涌[1] 李绍钦[2] 陈长广[2] 张同华[3] 

机构地区：[1]苏州大学附属第一医院心胸外科,215006 [2]苏州大学附属第一医院介入科,215006 [3]苏州大学附属第一医院放射科,215006

出　　处：《介入放射学杂志》2007年第4期266-268,共3页Journal of Interventional Radiology

基　　金：江苏省卫生厅重大课题(编号K200407)

摘　　要：目的探讨CT引导下经皮瘤块穿刺法制作兔VX2肺癌模型,并与开胸瘤块种植法进行比较。方法新西兰大白兔24只,随机分成两组,分别采用CT引导下经皮穿刺注射瘤组织块(A组,n=16)及开胸瘤组织块接种(B组,n=8)的方法种植于肺,肿瘤种植后14、21和28dCT平扫结合病检观察肿瘤大小及CT值。结果两组成瘤率均为100%,成瘤时间14～21d,A组CT示肺癌大小为(2.0±0.5)cm,CT值为(31±15)HU,手术操作时间为(15±3)min,肿瘤转移率为3/16,实验兔存活时间为(34±4)d。B组CT示肺癌大小为(1.9±0.5)cm,CT值为(29±16)HU,手术操作时间为(45±13)min,肿瘤转移率为6/8,实验兔存活时间为(27±4)d。两组存活时间,手术操作时间,肿瘤转移率差异有统计学意义(P<0.05)。结论CT引导下瘤块穿刺法建立兔VX2肺癌模型具有方法简单,成功率高,动物损伤小,转移率低等优点。Objective To compare the differences of VX2 lung carcinoma models in rabbits establishing via thoracotomic route and percutaneous puncture-inoculation method. Methods Twenty four New Zealand white rabbits were randomly divided into two groups. VX2 carcinoma tissue samples were injected into the lung under CT-guided percutaneus puncture-inoculation method in group A （n=16） and implanted into the lung via thoracotomic route in group B（n = 8）. 14, 21, 28 days later, plain CT scan and histopathologic test were carried out for assessment of the successful rates of the animal models and the condition of carcinoma. Results The successful rates of the two kinds of transplanted carcinoma were all 100%. The time of carcinoma growth was 14 - 21 days. In group A, the maximum carcinoma diameter was （2.0 ± 0.5） cm with CT value （31 ±15） HU, survival （34 ±4） days, operation time （15 ±3） min, and the metastasis rate of 18.75%（3/16）. In group B, the maximum carcinoma diameter was （1.9 ±0.5） cm with CT value （29 ±1,6） HU ,survival （27 ±4） days, operation time （45 ±13） min, and the metastasis rate of 75%（6/8）. The survival period operation time and the metastasis in group A were significantly lower than those in group B （P 〈 0.05）. Conclusion The CT-guided percutaneous puncture-inoculation method for establishing rabbit VX2 lung carcinoma is simple, high successful, mild damage and low metastasis. （J Intervent Radiol, 2007, 16： 266- 268）

关 键 词：肺 VX2肿瘤 动物模型 体层摄影术 X线计算机 

分 类 号：R734.2[医药卫生—肿瘤]