小鼠脑内注射Aβ_(25-35)记忆和M受体变化及梓醇的调节作用  

Changes of memory and M receptor injection of Aβ_(25-35) into basal ganglion region of forebrain and effect of catalpol

作　　者：刘硕[1] 胡雅儿[1] 夏宗勤[1] 

机构地区：[1]上海交通大学基础医学院细胞调控研究室,上海200025

出　　处：《上海交通大学学报（医学版）》2007年第8期945-948,共4页Journal of Shanghai Jiao tong University:Medical Science

基　　金：国家自然科学基金(30371636);上海市-联合利华研究与发展基金(06SU07006)~~

摘　　要：目的建立小鼠脑内定位注射淀粉样肽Aβ25-35和鹅蕈膏酸(IBO)造成的拟痴呆模型,并观察梓醇对于拟痴呆模型动物的短期记忆功能和脑M受体密度的不同影响。方法小鼠单侧脑基底核部位一次性定位注射淀粉样肽Aβ25-35和IBO造成拟痴呆模型后,设立梓醇用药组、模型组和对照组,冠状切片考察注射轨迹,Y-迷宫测定学习能力及放射配基结合分析法测定注射同侧脑内M胆碱受体密度,并用直线回归法检测两者的相关性。结果模型小鼠注射部位与图谱脑基底核部位一致,学习成绩和脑M受体密度明显低于对照组。连续2个月每天喂服梓醇50 mg/kg的小鼠学习和记忆能力显著好于模型组,脑M受体密度也显著提高,而且脑M受体密度与动物的学习记忆能力呈显著正相关。结论小鼠单侧脑基底核部位一次性定位注射淀粉样肽Aβ25-35和IBO可造成稳定的拟痴呆模型,梓醇能明显改善拟痴呆小鼠的短期记忆能力,同时能显著提高脑内M受体密度,两者的变化呈显著正相关。To establish mouse model of dementia by intracranial injection of Aβ25-35 and small amount of ibotenic acid （IBO） and to explore whether the effects of catalpol can affect the brain M receptor density and the short term memory. Methods The mice were randomly divided into three groups： control group,model group,treat group which were given orally for 2 months with 50 mg. kg^-1. d^-1 of catalpol. Dementia model was developed by single unilateral injection of 0.3 μL of a solution of Aβ25-35 normal saline containing 4 μg of Aβ25-35 and 1 μg of ibotenic acid） into right basal ganglion region according the atlas of mouse brain with the aid of a sterotaxic equipment. The track of injection was observed by HE staining. The learning/memory ability was measured by Y-maze performance. The brain muscarinic receptor density was analyzed with single-site binding assay using 3H-quinuclidinyl benzilae （QNB）. Results Two months after model development,the learning ability as well as the density of muscarinic receptor in brain were significantly decreased in model mice compared with those in control mice. Parallel models treated with daily oral administration of Catalpol for two months improved the learning ability and increased the brain muscarinic receptor density when compared with model mice. The correlation coefficient between total M receptor densities and the learning/memory ability was significant when examined with linear regresion. Conclusion A dementia model was established in mice. Dementia model was developed by single unilateral injection of 0.3 μL of a solution of Aβ （1 μL normal saline containing 4 μg of Aβ25-35 and I μg of ibotenic acid） into right basal ganglion region was established in mice. Catalpol can significantly improve the learning and increase the brain muscarinic receptor density of the model.

关 键 词：梓醇 M胆碱受体 放射配基受体结合分析 学习能力 拟痴呆模型 短期记忆 

分 类 号：R749.16[医药卫生—神经病学与精神病学]