枯草芽孢杆菌SN-02发酵液的抑菌谱及稳定性研究  

Antimicrobial Spectrum and Stability of the Fermentation Broth of Bacillus subtilis SN-02

作　　者：张崇[1] 赵秀香[1] 宋影[1] 高芬[1] 魏颖颖[1] 吴元华[1] 

机构地区：[1]沈阳农业大学植物保护学院,辽宁沈阳110161

出　　处：《生物技术》2007年第4期71-73,共3页Biotechnology

基　　金：辽宁省农业科技攻关招标项目资助(No.2004011)

摘　　要：目的：研究枯草芽孢杆菌SN-02发酵液的抑菌谱及稳定性。方法：以28种植物病原菌为供试菌,杯碟法测定SN-02菌发酵液的抑菌谱;以烟草靶斑病菌为指示菌,杯碟法测定发酵液的热稳定性、酸碱稳定性及传代稳定性。结果：SN-02菌发酵液对28种供试菌株的抑菌圈直径在20mm以上。将发酵液于120℃处理2.5h,-20℃处理25d抑菌活性没有明显变化;发酵液在pH 4-9时抑菌活性无明显变化,在pH 1-3和pH 10条件下抑菌活性明显下降;连续培养10代,发酵液抑菌活性没有下降。结论：SN-02菌发酵液抑菌谱较广,耐高温和低温,传代稳定性好,但在强酸和强碱条件下稳定性较差。Objective： Inhibitory spectrum and stability of Bacillus subtilis SN - 02 fermentation broth were studied. Methods： Cylinder - plate assay was used to mensurate inhibitory spectrum on 28 plant pathogens and to determine stability of the fermentation broth of Bacillus subtilis SN - 02 on Thanatephorus cumcumeris. Results：The inhibition diameters to 28 plant pathogens of the fermentation broth was up to 20mm.The fermentation broth could keep activity when treated at temperature 120℃ for 2.5h, - 20℃ for .25d and the pH value between 4 to 9, however, the inhibitory activity decreased obviously when pH value between 1 to 3 and 10. The inhibitory activity was not changed when the strain SN - 02 was subcultured for 10 generations. Conclusion： The inhibitory spectrum of strain SN- 02 fermentation broth was wide, stable to heat and low temperature, and good genetic stability, but the inhibitory activity fell down in extreme alkaline and extreme acid condition.

关 键 词：枯草芽孢杆菌 发酵液 抑菌谱 稳定性 

分 类 号：S476[农业科学—农业昆虫与害虫防治]