内皮细胞特异性分子-1在肺癌组织中的表达及其临床意义  

Expression of endocan and its clinical significance in lung cancer tissues

作　　者：徐大林[1] 桂淑玉[1] 李永怀[1] 谢斌[2] 周青[2] 丁向东[3] 汪渊[2] 

机构地区：[1]安徽医科大学第一附属医院呼吸内科,合肥230022 [2]安徽医科大学分子生物学实验室生物化学教研室和省部共建教育部重要遗传病基因资源利用重点实验室,合肥230032 [3]安徽医科大学病理解剖教研室,合肥230032

出　　处：《安徽医科大学学报》2007年第4期363-367,共5页Acta Universitatis Medicinalis Anhui

基　　金：安徽省自然科学基金资助项目(编号:050430705)

摘　　要：目的探讨内皮细胞特异性分子1(endocan)的表达与肺癌临床病理特征的关系及其临床意义。方法用免疫组化S-P法检测62例肺癌组织、15例良性肺疾病和5例正常肺组织中endocan的表达。结果endocan在肺癌组织血管内皮细胞中的高表达率70.97%(44/62),良性肺疾病和正常肺组织中血管内皮细胞的高表达率5.00%(1/20),差异有显著性(P<0.05);在有淋巴结转移的非小细胞肺癌组织中血管内皮细胞endocan高表达率88.89%(32/36),明显高于无淋巴结转移组的高表达率35.29%(6/17)(P<0.05);非小细胞肺癌(NSCLC)细胞胞质中endocan的高表达率为62.26%(33/53),且随分化程度降低而增高,高、中分化非小细胞肺癌细胞胞质的高表达率11.11%(2/18),低于低分化非小细胞肺癌细胞胞质的高表达率88.57%(31/35)(P<0.05)。而在小细胞肺癌组织细胞胞质中无表达。结论肺癌组织血管内皮细胞endocan的表达增高与淋巴结转移有关;非小细胞肺癌细胞胞质endocan的表达与肿瘤细胞分化有关。Objective To investigate the relationship between the expression of endocan and clinicopathological characteristics and and its clinical implication in lung cancer tissues . Methods The expression of endocan in tumoral and non-tumoral lung tissues was analyzed by immunohistochemlstry （ IHC ） （ labeled streptavidin-biotinmethod）. Results The high expression of endoean in the endothelia cell of lung cancer tissues was significantly higher than that in the non - tumoral lung tissues （70. 97 % and 5.00% , respectively） , P 〈 0. 05 ; The high expres-sion of endocan in the endothelia cell of NSCLC with lymph node involvements was higher than that without lymph node involvements（ 88.89% and 35.29% , respectively）, P 〈 0. 05 ;The high expression rate of endocan in the cytoplasm of NSCLC was 62. 26% , and the high expression of endocan in the cytoplasm of NSCLC with poor differentiation was significantly higher than that with well differentiation （88.57% and 11.11%, respectively）, P 〈 0. 05. But there was no expression of endocan in the cytoplasm of small cell lung cancer. Conclusions The high expression of endocan in the endothelia cell of lung cancer is related to lymph node involvements, and endocan expression in the cytoplasm of NSCLC is related to the tumoral cell differentiation.

关 键 词：肺肿瘤/病理学 免疫组织化学 肿瘤转移 

分 类 号：R734.2[医药卫生—肿瘤] R341[医药卫生—临床医学]