改良法大鼠酒精性脂肪肝模型的建立  

The improvement model of alcoholic fatty liver in rats

作　　者：王晓红[1] 曹琦[1] 李俊[1] 汪思应[2] 黄德武[2] 

机构地区：[1]安徽医科大学药学院,合肥230032 [2]安徽医科大学动物实验中心,合肥230032

出　　处：《安徽医科大学学报》2007年第4期420-423,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金资助项目(编号:30470775)

摘　　要：目的通过生理途径诱导大鼠酒精性脂肪肝模型。方法SD大鼠自由饮用酒精、蔗糖混合液体食料,12周后观察大鼠肝脏的组织形态学改变,血清中谷草转氨酶(ALT)、谷丙转氨酶(AST)活性和血浆中肿瘤坏死因子(TNFα-)水平以及肝组织中谷胱甘肽过氧化物酶(GSH-Px)、超氧化歧化酶(SOD)活性和丙二醛(MDA)含量。结果12周后酒精组大鼠ALT、AST活性、TNFα-含量和肝组织重MDA含量明显高于15%蔗糖组和正常组,而GSH-Px和SOD活性却低于15%蔗糖组和正常组,且肝组织出现大量脂肪变性,伴有局部炎症和坏死。结论采用生理途径诱导大鼠酒精性脂肪肝动物模型具有应用价值。Objective To establish the model of alcoholic fatty liver in rats fed alcohol by a physiological feeding way. Methods Male SD rats drunk the mixture of ethanol and sugar continuously for 12 weeks. The pathological changes of the liver was examined. Alanine aminotransferase （ALT）, aspartate transaminase （AST）, tumor necro- sis factor （TNF） -α in serum and glutathione peroxidase （ GSH-Px）, superoxide dismupase （SOD） and malondialdehyde（MDA） content in liver were assayed. Results 12 weeks after alcohol feeding, the levels of ALT, AST,TNF-α and MDA in model group were increased compared with those of the control group and normal group. GSH-Px and SOD activity in liver from model group were decreased compared with those of the control group and normal group. Moreover hepatic steatosis, local inflammation and necrosis were found in this model group. Conclusion The model of alcoholic fatty liver in rats fed alcohol by a physiological feeding way has an important effect.

关 键 词：脂肪肝 酒精性/病理学 疾病模型 动物 肿瘤坏死因子-Α 

分 类 号：R575.5[医药卫生—消化系统] R363-332[医药卫生—内科学]