CEBPs及其调节的基因与大鼠肝再生相关性分析  

Correlation analysis of CCAATI enhancer-binding proteins and their regulated genes with rat liver regeneration

作　　者：王望[1] 陈晓光[2] 徐存拴 

机构地区：[1]河南师范大学生命科学学院,河南省新乡市453007 [2]河南省科技部共建细胞分化调控重点实验室,河南省新乡市453007

出　　处：《世界华人消化杂志》2007年第20期2186-2193,共8页World Chinese Journal of Digestology

摘　　要：目的:探讨基因转录水平CCAAT增强子结合蛋白(CEBPs)及其调节基因在肝再生(LR)中的作用.方法:将与肝再生相关的CEBPs家族转录因子输入NCBI(www.ncbi.nlm.nih.gov)和RGD(rgd.mcw.edu)等网站查找与其转录功能相关的文献,从中得出大鼠、小鼠和人的CEBPs家族下游基因.然后将人和小鼠基因与大鼠比对,筛选出与大鼠不重复的人和小鼠基因.再将他们与Rat Genome 230 2.0芯片的检测结果进行比对确认,把其中表达上调或下调2倍以上的基因视为有意义变化的大鼠同源基因.用Rat Genome 230 2.0芯片检测他们在大鼠再生肝中的表达情况,用真、假手术比较方法确定肝再生相关基因.结果:27个基因与肝再生相关,其中11个基因表达上调,6个基因表达下调,10个基因在有的时点表达上调,有的时点表达下调(简称上/下调表达).他们的上调范围为对照的2-128倍,下调范围为对照的2-16倍.肝再生启动[部分肝切除(PH)后0.5-4 h]、G_0/G_1过渡(PH后4-6 h)、细胞增殖(PH后6-66 h)、细胞分化和组织结构功能重建(PH后72-168 h)等4个阶段起始表达的基因数分别为18,3,8和1;基因的总表达次数分别为18,11,25和16.表明相关基因主要在肝再生启动阶段起始表达,在不同阶段发挥作用.他们共表达上调126次,下调76次.表明肝再生中表达上调基因多于表达下调基因.结论:CEBPs及其调节的基因与肝再生中细胞增殖、细胞分化、细胞凋亡、炎症反应、应激反应、脂类代谢和细胞外基质变化等密切相关.AIM： To explore the effects on transcription levels of CCAAT/enhancer-binding proteins （CEBP） genes and genes regulated in liver regeneration （LR）. METHODS： CEBP family transcription factors correlated with LR were input into the websites of NCBI （www.ncbi.nlm.nih.gov） and RGD （rgd. mcw.edu）. Documents related to the transcrip- tion functions of CEBP family transcription factors were located and the CEBP family down- stream target genes in human, rat and mice ob- tained. The human and mice non-repeated genes in rat genes were screened out; the results were confirmed by comparison by Rat Genome 230 2.0 chip examination. Twice up-regulated and down-regulated genes were regarded as signifi- cantly changed rat homologous genes. The Rat Genome 230 2.0 chips were used to detect the expressions of the above genes in rat RL. Genes correlated with liver regeneration were confirmed by comparison in a true and false operation. RESULTS： Twenty-seven genes correlated with LR, 11 of which were up-regulated, 6 down-reg- ulated, and 10 that were up-regulated at certain time points but down-regulated at others. The up-regulated range was 2 to 128 fold of the com- parison group, while the down-regulated range was 2 to 16 fold. The number of genes expressed in the LR initiation [0.5-4 hours after partial hepatectomy （PH.）], G0/G1 transition （4-6 hours after PH）, cell proliferation （6-66 hours after PH） and cell differentiation and tissue structure reconstruction （72-168 hours after PH） stages were 18, 3, 8 and 1, respectively. Total expres- sion times were 18, 11, 25 and 16, respectively, showing that the correlated genes were primar- ily expressed in the LR initiation stage. Genes were up-regulated 126 fold and down-regulated 76 fold, indicating that more genes were up- regulated than down-regulated in LR. CONCLUSION： CEBPs and their regulated genes are highly correlated with cell proliferation, cell differentiation, cell apoptosis, inflammation, stress response, lipids metabolism and change

关 键 词：部分肝切除 大鼠基因组230 2.0芯片 CCAAT增强子结合蛋白 肝再生相关基因 

分 类 号：R575[医药卫生—消化系统]