抗突变中药对错配修复基因缺失结直肠癌细胞的抑制作用  

Inhibitory effect of Chinese herbs with anti-mutation activity on a mismatch-repairgene-deficient colorectal cancer cell line

作　　者：刘秀芳[1] 金黑鹰[1] 丁义江[1] 陆茵[2] 李璘[2] 丁曙晴[1] 刘飞[1] 倪敏[1] 王静[1] 

机构地区：[1]南京中医药大学第三附属医院全国肛肠医疗中心,江苏省南京市210001 [2]南京中医药大学海洋药物研究所,江苏省南京市210029

出　　处：《世界华人消化杂志》2007年第20期2201-2204,共4页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助课题;No.30572447;南京市医学发展重点项目资助课题;No.ZKX05028

摘　　要：目的:研究抗突变中药对错配修复基因缺失结直肠癌细胞的抑制作用.方法:使用具有抗突变作用的14种中药(冬凌草甲素、茶多酚、穿心莲内酯和黄芩苷等)处理hMSH2缺失结肠癌细胞株Lovo细胞和错配修复基因正常的结肠癌细胞株SW480细胞,采用MTT法观察细胞的增殖情况.多重荧光聚合酶链式反应(PCR)方法检测各浓度时Lovo细胞微卫星不稳定变化.结果:冬凌草甲素、茶多酚、穿心莲内酯和黄芩苷对Lovo细胞和SW480细胞有明显的抑制作用.冬凌草甲素、茶多酚和黄芩苷对Lovo细胞的抑制作用优于SW480细胞,特别是黄芩苷对Lovo细胞作用为SW480细胞的5倍,差异有显著意义.穿心莲内酯对SW480细胞的作用优于Lovo细胞,差异具有有显著意义.白藜芦醇、芦荟苷、丹参酮ⅡA、姜黄素4种中药成分作用于这2种细胞后,增殖抑制率较低,且加大药物浓度,增殖抑制率也无明显变化.仙灵脾等6种单味中药对2种细胞不但无明显抑制作用,反而显示有生长促进作用.另在各浓度,Lovo细胞微卫星均没有明显改变.结论:茶多酚和黄芩苷等具有抗突变作用的中药具有抑制错配修复基因缺失结直肠癌细胞的作用,其作用与剂量无关.AIM： To study the inhibitory effect of Chinese herbs with anti-mutation activity on the mismatch-repair-gene-deficient colorectal cancer cell line. METHODS： Fourteen Chinese herbs were used to treat the hMSH2-deficient colorectal cancer Lovo cell line and mismatch-repair-gene- deficient colorectal SW480 cell line, and growth rate was evaluated by the MTT method. The microsatellite instability change was examined by multiplex fluorescent polymerase chain reaction （PCR）. RESULTS： Tea polyphenols, orindonin, baicalin and andrographolide had obvious inhibition effects on colorectal cancer cell lines. The effects of orindonin, tea polyphenols and baicailin on the Lovo cell line were better than on SW480 cells. In particular, the effect of baicalin on Lovo cells was five times better than that on SW480 cells, but the effect of andrographolide on SW480 cells was better than that on Lovo cells. The proliferation rate of the two cell lines was low after resveratrol, aloin, tanshinone IIA and curcumin treatment, and increasing drug concentration had no effect. These results demonstrated that epimedium brevicornum and the other five drugs had no inhibitory effect on the two cell lines, but they did have a proliferative effect. The microsatellite instability had no changes in the different drug group concentrations. CONCLUSION： Chinese herbs with anti-mutation effects, such as orindonin, tea polyphenols and baicailin, exert an inhibitory effect on mismatch-repair-gene-deficient colorectal cancer cell lines. These effects are independent of the dose and duration of exposure. The mechanism may result from some yet unknown factors.

关 键 词：中药 抗突变作用 结肠癌 错配修复 MTT法 多重荧光聚合酶链式反应 体外实验 

分 类 号：R735.35[医药卫生—肿瘤]