Behet病患者结节红斑组织中CD40/CD40L共刺激分子的表达(英文)  

An increased expression of CD40/CD40L costimulatory molecules in erythema nodosum of patients with Behet's disease

作　　者：王红[1] 杨培增[1] 彭晓燕[2] 赵萌[2] 周红颜[1] 黄祥坤[1] 

机构地区：[1]中山大学中山眼科中心 [2]首都医科大学附属北京同仁医院眼科中心,中国北京市100730

出　　处：《国际眼科杂志》2007年第4期883-886,共4页International Eye Science

基　　金：中国国家自然科学基金资助项目(No.30400490);中国北京市自然科学基金资助项目(No.7052023);中国广东省中医药局资助项目(No.1050171)~~

摘　　要：目的:探讨Behet病患者结节红斑中CD40/CD40L分子的表达及意义。方法:采用免疫组化方法对5例Behet病活动期患者腿或臂部结节红斑中CD4,CD8,CD19,CD68,HLA-DR,CD40和CD40L分子表达的情况进行了检测,并用双重染色方法探讨了细胞来源。结果:Behet病结节红斑中表皮约90%的细胞表达CD40,但无CD40L表达。真皮和皮下表达CD4,CD8,CD19,CD68,HLA-DR,CD1a,CD40L和CD40的细胞均显著增多。双染结果显示,45%的CD4+T细胞表达CD40L分子。100%CD68+细胞表达CD40分子。59.2%的HLA-DR+细胞表达CD40分子。结论:CD40L分子主要表达于CD4+T细胞,CD40分子主要表达于单核巨噬细胞、抗原提呈细胞和角朊细胞。CD40/CD40L通路可诱导巨噬细胞和T淋巴细胞的功能和活性,对Behet病患者结节红斑的发生、炎症的持续和扩大起重要作用。AIM： To investigate the expression and the possible implication of CD40/CD40L costimulatory molecules in erythema nodosum of patients with Behcet＇s disease. METHODS： Sampling was done from erythema nodosum of 5 patients with Behcet＇s disease and normal skin of 2 healthy individuals. Immunohistochemical staining was performed to examine the expression of CD4, CD8, CD19, CD68, HLA-DR, CD40 and CD40L molecules in the obtained tissues. RESULTS： Approximately 90% of epidermic ceils in erythema nodosum expressed CD40 molecule. In the dermis and subcutaneous tissue, a significantly increased number of CD4^＋Tcells, CD8^＋Tcells, CD19^＋cells, CD68^＋cells, HLA-DR^＋cells, CD40L^＋cells, and CD40^＋cells were observed in the erythema nodosum as compared with that in normal skin. Double staining showed that CD40L molecules were expressed on 45% of CD4^＋ T cells. CD40 molecules were expressed on 1000 CD68^＋ cells and 59.2% of HLA-DR^＋cells respectively. CONCLUSION： A number of CD40/CD40L costimulatory molecules are upregulated in the erythema nodosum of patients with Behcet＇s disease.

关 键 词：BEHCET病 结节红斑 共刺激分子 

分 类 号：R771.3[医药卫生—眼科]