汉防己甲素对翼状胬肉成纤维细胞基质金属蛋白酶的影响  

Inhibitory effect of tetrandrine on matrix metalloproteinases in pterygium fibroblasts in culture

作　　者：安美霞[1] 吴开力[2] 林少春[2] 胡世兴[2] 

机构地区：[1]中国广东省中山市小榄人民医院眼科,528415 [2]中山大学中山眼科中心中山大学眼科学国家重点实验室,中国广东省广州市510060

出　　处：《国际眼科杂志》2007年第4期938-940,共3页International Eye Science

基　　金：中国广东省科技重点攻关项目(No.2KM04102S);广州市科技计划项目基金资助(No.2007J1_C0101)~~

摘　　要：目的:观察汉防己甲素(Tet)对翼状胬肉成纤维细胞基质金属蛋白酶(MMP)分泌及表达的影响。方法:收集正常球结膜与初发翼状胬肉成纤维细胞培养的上清液,ELISA法检测两种细胞培养上清液中MMP-1,MMP-3,MMP-9的含量及10-5mol/L的Tet作用48h翼状胬肉成纤维细胞培养的上清液中含量的变化。荧光定量PCR检测10-5mol/L的Tet对翼状胬肉成纤维细胞MMP-3mRNA表达的影响。结果:培养的初发翼状胬肉成纤维细胞上清中MMP-1,MMP-3含量较正常成纤维细胞上清中含量高[MMP-1:26928±1135→3148±74(ng/L),MMP-3:52593±1031→12490±3126(ng/L),P<0.01],加入10-5mol/LTet作用48h后明显下降[MMP-1:26928±1135→15064±533(ng/L),MMP-3:52593±1031→29943±561(ng/L),P<0.01]。培养的正常结膜及初发翼状胬肉成纤维细胞上清中未检测到MMP-9。初发翼状胬肉成纤维细胞MMP-3mRNA是正常结膜组织成纤维细胞的3倍,10-5mol/L的Tet作用24h后表达量下降了27.1%。结论:Tet对翼状胬肉成纤维细胞MMP-1,MMP-3的分泌有明显的抑制作用,并能抑制其细胞MMP-3mRNA表达量。AIM： To examine the changes of matrix metalloproteinases （MMPs） in medium secreted by primary human pterygium fibroblasts （HPF） and the expression patterns of MMP in cultured HPFs affected by tetrandrine （Tet）. METHODS： Human conjunctival fibroblast （HCF） and HPF were cultured at medium containing RPMI 1640 under the same conditions. The changes of protein levels of MMP-1, MMP-3 and MMP-9 were determined by Enzyme-linked immunosorbent assay （ELISA） respectively when treated with 10^-5mol/L Tel： for 48 hours in a serum-free medium. The changing expression of transcripts of MMP-3 mRNA in HPFs was studied by real-time fluorescent quantitative PCR method. RESULTS： The protein levels of MMP-1 and MMP-3 were higher in a serum-free medium in cultured HPFs compared to normal HCFs （P〈0.01）. The amount of MMP-1 and MMP-3 in the cultured HPF conditioned medium was significantly decreased by addition of 10^-5mol/L Tet for 48 hours （P〈 0.01）. MMP-9 protein was not found in HPF or HCF conditioned medium. Expression of MMP-3 mRNA in the cultured HPFs was more dramatically increased compared with the normal HCF （3-fold increase） and decreased by 27.1% when treated by 10^-5mol/L Tet for 24 hours. CONCLUSION： The 10^-5mol/L Tet can dramatically decrease MMP-1 and MMP-3 proteins secreted by cultured HPFs and expression of transcripts of MMP-3 mRNA in HPFs.

关 键 词：汉防己甲素 翼状胬肉 基质金属蛋白酶 

分 类 号：R777.33[医药卫生—眼科]