HPLC-UV-ELSD法同时测定青蒿中青蒿素、青蒿乙素和青蒿酸的含量  

Determination of artemisinin,arteannuin B and artemisinic acid in Herba Artemisiae Annuae by HPLC-UV-ELSD

作　　者：张东[1] 杨岚[1] 杨立新[1] 王满元[2] 屠呦呦[1] 

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]首都医科大学中医药学院,北京100069

出　　处：《药学学报》2007年第9期978-981,共4页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金项目(30572319)资助.

摘　　要：用HPLC-UV-ELSD法同时测定青蒿药材中青蒿素、青蒿乙素和青蒿酸的含量。采用Nucleodur C18色谱柱（250mm×4．6mm，5μmID）；以乙腈-0．1％乙酸水（50：50）为流动相；紫外检测波长209nm，蒸发光散射检测器漂移管温度50℃。结果显示，青蒿素、青蒿乙素和青蒿酸能够达到很好分离。它们的线性范围分别为0．52．2．6μg，r=0.9994（n=5）；0．022～4．4μg，r=0．9999（n=5）；0．203～8．12μg，r=0．9998（n=5）。平均回收率分别为99．45％（RSD=2．3％，n=6）；102．37％（RSD=1．7％，n=6）；101．10％（RSD=0．79％，n=6）。本法简单、准确、快速，可同时测定青蒿药材中青蒿素、青蒿乙素和青蒿酸的含量。To establish an HPLC-UV-ELSD method for the determination of the content of artemisinin, arteannuin B and artemisinic acid in Herba Artemisiae Annuae. The analytical column was Nucleodur RP-C17 （250 mm × 4.6 mm, 5 μm ID）. The mobile phase was acetonirile-O. 1% acetic acid （50： 50） and the flow rate was 1.0 mL ·min^-1 with a UV detector for artemisinin, the detection wavelength at 209 nm, and the evaporative light-scattering detector （ELSD） for arteannuin B and artemisinic acid, the drift tube temperature： 50℃ ,the nitrogen flow rate 30 psi and the gain was 50. The resolution of artemisinin, arteannuin B and artemisinic acid was good. The linear calibration curves were obtained over the range of 0.52-2.6μg for artemisinin （r =0.999 4, n =5）, 0.022-4.4 μg for artemisinin B （r=0.999 9, n=5） and 0.203-8.12 μg for artemisinic acid （r=0.999 8, n=5）, separately. The mean recoveries of the three compounds were 99.45% , 102.37% and 101.10% with RSD of 2.3% , 1.7% and 0.79% , respectively. This method is simple, rapid, accurate and suitable for the determination of the content of the three compounds in the herbs.

关 键 词：青蒿 HPLC-UV-ELSD 青蒿素 青蒿乙素 青蒿酸 

分 类 号：R917[医药卫生—药物分析学]