5个虹鳟群体的生化遗传分析  

Biochemical genetics among 5 populations of rainbow trout

作　　者：庞艳红[1] 孙中武[1] 尹洪滨[2] 王炳谦[2] 

机构地区：[1]东北林业大学生命科学学院,黑龙江哈尔滨150040 [2]中国水产科学研究院黑龙江水产研究所,黑龙江哈尔滨150070

出　　处：《水产学报》2007年第4期539-544,共6页Journal of Fisheries of China

基　　金：国家技术基础条件平台建设项目(2004DKA30470-005);国家科技攻关项目(2004BA526B0111)

摘　　要：The levels of genetic variation at 40 genic loci of 15 isozymic systems in rainbow trout(Oncorhynchus mykiss) were determined among Donaldsons super rainbow trout,rainbow trout from California of USA,rainbow trout from Norway, rainbow trout from Danmark,and Jalo rainbow trout respectively.The genetic parameters would be important in selective breeding programs.As a main research means,the discontinuous vertical plate polyacrylamide gel electrophoresis(PAGE) was used to analyze the biochemical genetics status of 5 populations of rainbow trout.The P of 5 populations of rainbow trout ranged from 43.24% to 47.37%;the He′ of 5 populations of rainbow trout ranged from 0.1917 to 0.2178;the Ho′ ranged from 0.2703 to 0.3291;the D ranged from 0.4100 to 0.5331;the Ne ranged from 1.4865 to 1.5385.The chi-Square test showed all polymorphic loci from the 5 populations of rainbow trout were in Hardy-Weinberg equilibrium.Various genetic indexes showed that resource quality of the 5 populations of rainbow trout resource quality was still good.Phylogenetic tree showed rainbow trout from Norway,rainbow trout from Danmark,rainbow trout from California of USA and Donaldsons super rainbow trout belonged to the same clade,and Jalo rainbow trout belonged to another clade.The levels of genetic variation at 40 genic loci of 15 isozymic systems in rainbow trout（ Oncorhynchus mykiss ） were determined among Donaldsons super rainbow trout, rainbow trout from California of USA, rainbow trout from Norway, rainbow trout from Danmark, and Jalo rainbow trout respectively. The genetic parameters would be important in selective breeding programs. As a main research means, the discontinuous vertical plate polyacrylamide gel electrophoresis （PAGE） was used to analyze the biochemical genetics status of 5 populations of rainbow trout. The P of 5 populations of rainbow trout ranged from 43.24 % to 47.37 % ; the He′ of 5 populations of rainbow trout ranged from 0. 1917 to 0. 2178; the Ho＇ ranged from 0. 2703 to 0. 3291; the D ranged from 0. 4100 to 0. 5331; the Ne ranged from 1. 4865 to 1. 5385. The chi-Square test showed all polymorphic loci from the 5 populations of rainbow trout were in Hardy-Weinberg equilibrium. Various genetic indexes showed that resource quality of the 5 populations of rainbow trout resource quality was still good. Phylogenetic tree showed rainbow trout from Norway, rainbow trout from Danmark, rainbow trout from California of USA and Donaldsons super rainbow trout belonged to the same clade, and Jalo rainbow trout belonged to another clade.

关 键 词：虹鳟 群体 同工酶 遗传结构 遗传变异 

分 类 号：S917[农业科学—水产科学]