射频消融联合亚砷酸治疗兔肝VX2肿瘤的实验研究  

Experimental study of radiofrequency ablation combined with arsenious acid therapy on liver VX2 tumors in rabbits

作　　者：张盟辉[1] 王巧玲[2] 孔宪炳[3] 宋瑞卿 甘兆红[1] 樊伟[1] 王辉[1] 

机构地区：[1]山东省济南市第四人民医院普通外科,山东济南250031 [2]山东省济南市第二人民医院外科,山东济南250001 [3]重庆医科大学附属第一医院肝胆外科,重庆400016 [4]山东省鄄城人民医院外科,山东鄄城274500

出　　处：《中国普通外科杂志》2007年第8期770-773,共4页China Journal of General Surgery

摘　　要：目的探讨射频消融联合亚砷酸局部治疗对兔肝VX2肿瘤的有效性和安全性,并探讨其机制。方法建立新西兰兔肝VX2肿瘤模型28只,随机分为4组,每组7只,分别进行亚砷酸(亚砷酸组)、射频消融(射频组)、射频消融加亚砷酸(联合组)和生理盐水(对照组)治疗。于术前、术后1,3,7d和14d耳缘静脉抽血检查丙氨酸转氨酶(ALT),同时行超声学检查。治疗后第14天处死所有实验动物,通过免疫组织化学方法检测VEGF,并分析其与肿瘤体积的相关性。结果联合组治疗后仅第1天ALT与术前差异有显著性(P<0.05),第3天下降至接近术前水平;联合治疗能更好地抑制肿瘤生长;亚砷酸组、射频组及联合组肿瘤组织VEGF水平均低于对照组(P<0.05),射频组低于亚砷酸组(P<0.05),联合组低于其余2组,VEGF表达强度与肿瘤体积呈显著正相关(r=0.78,P<0.01)。结论联合治疗能通过抑制肿瘤新生血管生成而抑制肿瘤生长,且对肝脏无明显损害。Objective To evaluate the safety and efficacy of combining radiofrequency ablation （ RFA ） with arsenious acid （AA） locally to treat liver VX2 tumor in rabbits, and to explore its mechanism. Methods Twenty-eight New Zealand White rabbits with implanted liver VX2 tumors were randomly divided into four groups ： control group （ n = 7 ） , AA group （ n = 7 ） , RFA group （ n = 7 ） , and combination （ RFA ＋AA） group （n=7）. ALT was measured before treatment and on the day 0, day 3, day 7 and day 14 after treatment, meanwhile ultrasonic （US） examination was performed. All rabbits were killed 14 days after treatment. Vascular endothelial growth factor （VEGF） was examined by immunohistochemistry, and the relationship of VEGF and gross tumor volume was analyzed. Results Combination （ RAF ＋ AA ） therapy caused little damage to hepatic function but had better inhibited tumor growth. The level of VEGF in AA, RFA and RAF ＋ AA group was lower than that of the control group （ P 〈 0.05 ） , The level of VEGF in the RFA group were lower than the AA group （ P 〈 0. 05 ） while in the RAF ＋ AA group it was the lowest, Conclusions Combined （RAF ＋ AA ） therapy can inhibit VEGF expression which can inhibit tumor growth and with no significant liver damage.

关 键 词：肝肿瘤/治疗 射频消融 亚砷酸 血管内皮生长因子 疾病模型 动物 

分 类 号：R735.7[医药卫生—肿瘤]