TGF-β1在大肠癌发病机制中的作用  

The role of TGF-β1 in the pathogenesis of colon cancer

作　　者：黄志刚[1] 冉志华[2] 陈锡美[1] 萧树东[2] 陆玮[3] 

机构地区：[1]同济大学附属同济医院消化科,上海200065 [2]上海交通大学附属仁济医院消化科,上海200001 [3]复旦大学附属华山医院消化科,上海200040

出　　处：《同济大学学报（医学版）》2007年第4期17-20,共4页Journal of Tongji University(Medical Science)

摘　　要：目的通过RT-PCR方法观察TGF-β1在大肠癌组织中的mRNA表达及RNA干扰阻断TGF-β1表达对大肠癌细胞生长周期和侵袭力的影响。方法收集20例各期大肠癌手术标本抽提RNA,用半定量RT-PCR方法检测各组织中TGF-β1的mRNA表达。用小片段TGF-β1的双链RNA（siRNA）转染大肠癌HCT116细胞,半定量RT-PCR检测TGF-β1mRNA受抑程度,流式细胞仪观察转染后细胞周期变化,软琼脂克隆实验检测肿瘤细胞生长及成瘤性的变化。结果在20例各期大肠癌中TGF-β1检测12例（60%）存在mRNA过度表达。小片断RNA转染显著抑制了肿瘤细胞TGF-β1的表达,使细胞HCT 116的S期细胞较对照组明显增高59%-82.5%,进入G2期细胞减少;转染组细胞克隆形成率较对照组显著下降。结论TGF-β1mRNA在大肠癌组织中过度表达,通过RNA干扰技术可以快捷方便的阻断TGF-β1mRNA表达,使大肠癌细胞S期阻滞并抑制肿瘤细胞生长、降低其成瘤性。Objective To observe TGF-β1 mRNA expression in the tissue of colon cancer and the effects of inhibition of TGF-β1 on the growth and aggression of colon cancer cells. Methods The specimens of 20 patients with colon cancer were collected and total RNA was extracted for RT-PCR detection. HCT 116 colon cancer cells were transfected with small interfering dsRNAs（siRNA）. The effects of the inhibition of TGF-β1 were tested by semi-quantitative RT-PCR and changes in cell cycle distribution were analyzed by flow cytometry, while the ability of aggression was detected by soft agar colony assay. Results The mRNA expressions of TGF-β1 were over-expressed in 12/20 （60%） colon cancer tissues. The inhibition of the expression of TGF-β1 was significantly different after the transfection of siRNA against TGF-β1, and this induced the increase in S phase by 59 % -82.5 % and decrease in G2 phase, colony assay further demonstrated that siRNA against TGF-β1 led to a significant reduction in colony formation as compared with that of the control group. Conclusion The results suggest that mRNA over-expression of TGF-β1 is found in patients with colon cancer. And mRNA expression of TGF-β1 in the colon cancer cells is conveniently blocked by siRNA transfection.

关 键 词：TGF-Β1 大肠肿瘤 RNA干扰 半定量RT-PCR 

分 类 号：R574.6[医药卫生—消化系统]