腺病毒介导IL-24联合化疗药物增强对肝癌细胞PLC/PRF/5增殖的抑制  

Enhanced inhibitory effect of adenovirus-mediated IL-24 combined with chemotherapy agent on growth of liver cancer cell line PLC/PRF/5

作　　者：温莹浩[1] 殷正丰[1] 康晓燕[1] 李瑾[1] 钱海华[1] 陈玮[1] 

机构地区：[1]第二军医大学东方肝胆外科医院分子肿瘤实验室,上海200438

出　　处：《中国肿瘤生物治疗杂志》2007年第4期338-341,共4页Chinese Journal of Cancer Biotherapy

基　　金：国家自然科学基金项目(No30500477)~~

摘　　要：目的:研究腺病毒介导IL-24基因(Ad.IL-24)与化疗药物联用对肝癌细胞株PLC/PRF/5增殖的抑制作用。方法:用Ad.IL-24分别联合化疗药物氟尿嘧啶(fluorouracil,5-Fu)和表柔比星(epirubicin,EPI)处理培养的肝癌细胞株PLC/PRF/5,MTT法检测细胞增殖抑制率,流式细胞术(FCM)检测细胞周期和凋亡率。结果:10 MOI Ad.IL-24与25μg/ml5-Fu联合应用后72h,PLC/PRF/5细胞增殖抑制率达(67.4±0.58)%,显著高于单用Ad.IL-24组的(46.8±0.74)%和5-Fu组的(29.3±0.60)%(均P<0.05);10MOIAd.IL-24与2.5μg/mlEPI联合应用后72h,PLC/PRF/5细胞增殖抑制率达(72.5±0.92)%,显著高于单用Ad.IL-24组的(46.8±0.74)%和EPI组的(32.2±0.69)%(均P<0.05)。流式细胞术检测结果显示,Ad.IL-24与5-Fu或EPI联合应用明显导致细胞在G2/M期阻滞;Ad.IL-24+5-Fu组细胞凋亡率为(52.15±2.32)%,显著高于单用Ad.IL-24组的(28.36±3.49)%和5-Fu组的(8.27±2.61)%(均P<0.05);Ad.IL-24+EPI组细胞凋亡率为(58.67±1.73)%,显著高于单用Ad.IL-24组的(28.36±3.49)%和EPI组的(11.82±1.91)%(均P<0.05)。结论:Ad.IL-24与5-Fu或EPI联用能显著提高对肝癌细胞株PLC/PRF/5增殖的抑制作用。Objective： To investigate the inhibitory effect of adenovirus-mediated IL-24 （ Ad. IL-24） combined with fluorouracil （5-Fu） or epirubicin （EPI） on growth of human liver cancer cell line PLC/PRF/5. Methods： Human liver cancer cell line PLC/PRF/5 was treated with Ad. IL-24 combined with 5-Fu or EPI separately. The growth inhibition rate of cells was analyzed by MTY assay ; the cell cycle and apoptosis were detected by flow cytometry （FCM）. Results： Three days after combined treatment with Ad. IL-24（ 10 MOI ） and 5-Fu（25 μg/ml）, the growth inhibitory rate of PLC/PRF/5 cells was （67.4 ± 0.58 ） %, which was significantly higher than that in Ad. IL-24 group （ [ 46.8 ± 0.74 ] %, P 〈 0. 05 ） and 5-Fu group （ [ 29.3 ± 0.60 ] %, P 〈 0.05 ） ; three days after combined treatment with Ad. IL-24 （ 10 MOI ） and EPI （ 2.5 μg/ml）, the growth inhibitory rate was （72.5 ±0. 92 ） %, which was also significantly higher than those in Ad. IL- 24 group （ [ 46.8 ±0.74 ] %, P 〈 0.05 ） and EPI group （ [ 32.2 ± 0.69 ] %, P 〈 0.05 ）. FCM showed that combined administration of Ad. IL-24 and 5-Fu or EPI remarkably arrested PLC/PRF/5 cells at G2/M phase. The cell apoptotic rate was （52. 15±2.32）% in Ad. IL-24 ＋5-Fu group, which was significantly higher than those in Ad. IL-24 group （[28.36±3.491%, P〈0.05） and 5-Fu group （[8.27 ±2.611%, P〈0.05）. The cell apoptotic rate of was （58.67 ± 1.73） % in Ad. IL-24 ＋ EPI group, which was significantly higher than those in Ad. IL-24 group （ [ 28.36 ± 3.49 ] %, P 〈 0.05） and EPI group （ [ 11.82 ±1.91 ] %, P 〈 0.05 ）. Conclusion： Ad. IL-24 combined with 5-Fu or EPI has enhanced inhibitory effect on growth of liver cancer cell line PLC/PRF/5.

关 键 词：白介素24 肝肿瘤 基因治疗 化学疗法 表柔比星 氟尿嘧啶 

分 类 号：R730[医药卫生—肿瘤]