β-胡萝卜素对促癌剂抑制细胞间隙连接通讯的拮抗作用及机理  

Protective Effects and Mechanisms of β carotene on Inhibition of Gap Junctional Intercellular Communication by Tumor Promoter

作　　者：朱俊东[1] 糜漫天[1] 陈东升[1] 杨家驹[1] 石元刚[1] 

机构地区：[1]第三军医大学营养卫生学教研室

出　　处：《中国公共卫生学报》1997年第2期76-78,共3页

基　　金：国家自然科学基金

摘　　要：应用划痕标记染料示踪技术检测β－胡萝卜素对促癌剂ＴＰＡ抑制ＮＩＨ／３Ｔ３细胞间隙连接通讯（ＧＪＩＣ）功能的影响，同时对细胞膜流动性和细胞膜唾液酸含量的变化与ＴＰＡ抑制细胞ＧＪＩＣ功能的关系，以及β－胡萝卜素对这些变化的作用进行了研究。结果显示：β－胡萝卜素对ＮＩＨ／３Ｔ３细胞ＧＪＩＣ功能有促进作用，并对ＴＰＡ抑制ＮＩＨ／３Ｔ３细胞ＧＪＩＣ功能有一定的拮抗作用，随剂量升高上述作用表现为增强趋势；ＴＰＡ抑制细胞ＧＪＩＣ功能的同时显著升高膜流动性和膜唾液酸含量，而β－胡萝卜素对ＴＰＡ诱导的这些效应有拮抗作用。The effects of β carotene on the inhibition of gap junctional intercellular communication (GJIC) between NIH/3T3 cells by tumor promoter TPA and roles of membrane fluidity and membrane sialic acid were studied.The results showed that β carotene promoted GJIC between normal NIH/3T3 cells and protected the inhibition of GJIC by TPA in a dose dependent manner.In TPA group,the membrane fluidity and the membrane sialic acid increased siginificantly compared with control group.The above two indices in β carotene plus TPA group decreased siginificantly compared with TPA group.These results suggested that β carotene could protect the inhibition of GJIC by TPA,and the protective effects may be correlated with the changes of membrane fluidity and membrane sialic acid.

关 键 词：胡萝卜素 促癌剂 膜流动性 唾液酸 细胞间通信 

分 类 号：R730.1[医药卫生—肿瘤]