槲皮素对脑缺血再灌后FADD蛋白表达的影响  

Effects of Quercetion on FADD protein expression following focal cerebral ischemia/reperfusion

作　　者：吴志国[1] 毕方方[1] 杨晓苏[1] 肖波[1] 

机构地区：[1]中南大学湘雅医院神经内科,410008

出　　处：《神经疾病与精神卫生》2007年第1期1-2,6,共3页Journal of Neuroscience and Mental Health

基　　金：国家自然科学基金(30370514);教育部高校青年教师奖(2001-182)

摘　　要：目的研究大鼠脑缺血/再灌后Fas相关死亡域蛋白（FADD）的表达及槲皮素对其影响。方法用免疫组化法测定缺血2h再灌注不同时相缺血半暗带FADD蛋白的表达。结果缺血半暗带脑皮质内FADD蛋白的表达于再灌注3h明显升高,再灌注12h达高峰（P〈0.01）,至再灌注24h其表达明显下降。槲皮素能显著下调其表达（P〈0.01-0.05）。结论脑缺血/再灌后缺血半暗带FADD蛋白表达明显增加,提示可能在脑缺血/再灌注损伤中发挥重要作用。槲皮素可能通过抑制其表达从而达到脑保护的作用。Objective To study the protein expression of Fas--associated with death domain protein（FADD）, and the effect of Quercetin on them. Methods The rats were subjected to the middle cerebral artery occlusion for two hours, then killed at different time points after reperfusion. The protein expression of FADD were measured by immunohistochemistry. Results The protein expression of FADD were markedly increased at 3 h after reperfusion in the penumbra of rat cortex, and peaked at 12 h,then declined quickly at 24. Quercetin treatment could markedly downregulate the FADD protein expression after 3 h of reperfusion. Conclusions The protein expression of FADD increased in the penumbra of rat cortex after transient focal cerebral ischemia , which suggests that they may play an important role in the cerebral ischemia and reperfusion injury. Quercetin could protect neurons from ischemic damage through decreasing the expression level of FADD.

关 键 词：脑缺血 再灌注 FADD 槲皮素 

分 类 号：R743.3[医药卫生—神经病学与精神病学]