脑室内低温对家兔急性大脑中动脉缺血模型HSP70表达的影响  

The effects of cerebral ventricle low temperature on the expression of heat shock protein 70 in middle cerebral artery occlusion (MCAO) rabbits

作　　者：姚冰[1] 姚长义[1] 

机构地区：[1]中国医科大学附属第一医院神经外科,110001

出　　处：《神经疾病与精神卫生》2007年第2期98-100,共3页Journal of Neuroscience and Mental Health

基　　金：辽宁省自然科学基金(20042069)

摘　　要：目的观察脑室内低温对家兔急性大脑中动脉缺血模型热休克蛋白70((Heat shock protein70,HSP70)表达的影响。方法家兔16只,随机分为2组:对照组(常温生理盐水)、低温组(22℃低温生理盐水)各8只,应用线栓法建立大脑中动脉闭塞(MCAO)模型,进行脑室内穿刺低温液体灌注亚低温干预,24h后采用SABC方法检测各组脑组织HSP70表达,TUNEL方法检测凋亡细胞。结果低温组的TUNEL染色阳性细胞数和HSP70染色阳性细胞数明显低于及多于常温组,有显著性差异(P<0.05)。结论通过脑室内低温治疗,可以减少缺血区域凋亡细胞,并增加HSP70细胞的表达。同时改善了动物的神经系统症状。Objective To explore the protective effects of continuous trickle of low temperature liquids though the lateral ventricle on heat shock protein 70 expression after middle cerebral artery occlusion（MCAO） in rabbits. Methods 16 rabbits were randomly divided into the control group （normal temperature liquid） n =8 and the mild hypothermia group （22℃ low temperature liquid） n =8 after MCAO. The middle cerebral artery occlusion model is made by intralumial thread approach. Then we trickled the lateral ventricle continuously with normal temperature and low temperature liquids. After 24 hours, we observe the pathology of ischemic of MCAO. SABC immunohistochemistry was employed to detect the expression of HSP70 and TUNEL assay was used to detect the apoptotic cells. Results The apoptotie cells number in the mild hypothermia group were much lower than those of the control group and the expression of HSP70 increased in the mild hypothermia group, with statistical significance （P〈 0.05） . Conclusions Continuous trickle of low temperature liquids though the lateral ventricle may decrease the number of the apoptotic cells and increase the expression of HSP70, and it may improve the animal＇s nervous system function.

关 键 词：脑室 亚低温 脑梗死 HSP70 

分 类 号：R743[医药卫生—神经病学与精神病学]