一氧化氮在埃索美拉唑对大鼠胃黏膜损伤保护中的作用  

Role of nitric oxide in the protective effect of esomeprazole on gastric mucosal lesion in rats

作　　者：乐桥良[1] 张志坚[1] 林克荣[1] 

机构地区：[1]南京军区福州总医院消化科,福建福州350025

出　　处：《第四军医大学学报》2007年第17期1573-1575,共3页Journal of the Fourth Military Medical University

摘　　要：目的:探讨一氧化氮(NO)在埃索美拉唑对大鼠胃黏膜损伤保护中的作用.方法:在乙醇诱导大鼠胃黏膜损伤前,预先给予埃索美拉唑(20mg/kg)灌胃,L-硝基-精氨酸甲酯(L-NAME,4mg/kg)、L-精氨酸(250mg/kg)及D-精氨酸(250mg/kg)静脉注射.采用激光多普勒血流计(LDF)测定胃黏膜血流量(GMBF),镉粒还原和比色法测定胃黏膜和血浆NO2-/NO3-含量,并观察了胃黏膜损伤指数(UI)、溃疡坏死组织和中性粒细胞浸润严重程度的变化.结果:与模型损伤组比,埃索美拉唑组大鼠UI降低(P<0.01),溃疡坏死组织和中性粒细胞浸润程度减轻(P<0.01).预先用L-NAME处理后,埃索美拉唑保护胃黏膜损伤作用减弱;L-NAME抑制作用可被L-精氨酸拮抗,而不被D-精氨酸拮抗.向胃内灌注埃索美拉唑,可增加GMBF,胃黏膜和血浆NO2-/NO3-,L-NAME可逆转这种作用,但对埃索美拉唑抑制酸分泌作用无明显影响.结论:埃索美拉唑对大鼠胃黏膜损伤保护作用与NO有关,这一作用与抑制胃酸分泌无关.AIM： To observe the role of nitric oxide （NO） in the protective effect of esomeprazole on the gastric mucosal lesion in rats. METHODS： Before gastric mucosal lesion was made in rats by pure alcohol, esomeprazole （20 mg/kg） was administered into the gastric chamber and N -nitro-L-arginine methyl ester （ L- NAME, 4 mg/kg ）, L-arginine （ 250 mg/kg ） and D-arginine （250 mg/kg） were injected intravenously. Gastric mucosal blood flow （GMBF） was assessed with laser Doppler flowmetry （LDF）, gastric mucosal and serum NO2^-/NO3^- were measured by cadmium granulation reduction and colorimetric method, and the changes of ulcer index and the severity of tissues necrosis and neutrophils infiltration were observed. RESULTS： Ulcer index of the esomeprazole group was much lower than that of the control group （ P 〈 0.01 ）, and the degree of tissues necrosis and neutrophils infiltration were much milder than those of the control group （P 〈 0.01 ）. The protective effect of esomeprazole was significantly decreased by prior administration of L-NAME. The inhibitory effect of L-NAME was antagonized by prior administration of L-arginine, but not D-arginine. Esomeprazole administered into the gastric chamber obviously increased GMBF, gastric mucosal and serum NO2-/NO3-, which was prevented by pretreatment with L-NAME, but the antisecretory effect of esomeprazole was not affected by L-NAME. CONCLUSION： Esomeprazole has some NO-mediated protective effect against gastric mucosal lesion in rats and the action of esomeprazole against gastric acid secretion contributes little to the protective effect.

关 键 词：一氧化氮 埃索美拉唑 胃黏膜损伤 细胞保护 大鼠 

分 类 号：R573.1[医药卫生—消化系统]