多西他赛对乳腺癌细胞中TP酶的调节及其与化疗敏感性的研究  

Regulation of thymidine phosphorylase level in breast carcinoma cells by docetaxel and its relationship with the sensitivity to the prodrug of 5-fluorouracil in vitro

作　　者：姜玉石[1] 邹庆华[1] 

机构地区：[1]天津医科大学附属石油医院普外科,河北廊坊065000

出　　处：《第四军医大学学报》2007年第17期1580-1582,共3页Journal of the Fourth Military Medical University

摘　　要：目的:探讨多西他赛对乳腺癌细胞中胸苷磷酸化酶(TP酶)的调节作用及其与化疗敏感性的关系.方法:应用多西他赛诱导乳腺癌细胞中的TP酶,酶联免疫吸附法(ELISA)检测肿瘤组织中TP酶蛋白水平,采用MTT法检测药物敏感性变化.结果:乳腺癌细胞株MCF-7中TP酶含量为(30.1±1.4)μg/g,乳腺癌耐药株MCF/ADR的TP酶含量为(18.8±0.4)μg/g.多西他赛能上调MCF-7和MCF/ADR中TP酶表达,且具有时间、剂量双重依赖性.耐药株中TP酶上调最高达(30.0±1.2)μg/L,未能达到非耐药细胞株水平.MCF-7细胞中TP酶上调1.35倍,对氟铁龙的敏感性提高了2.06倍,有统计学意义.结论:多西他赛能上调乳腺癌细胞TP酶,呈剂量-时间依赖性.AIM： To induce thymidine phosphorylase （TP） in breast carcinoma cells with docetaxel to increase its sensitivity to fluoropyridine drugs. METHODS： The TP level in tumor cells was measured by ELISA. And then the assay was done with MTT staining for the cells treated with small concentration Docetaxel. RESULTS： The rip level was significantly higher in MCF-7 [ （30.1± 1.4） μg/g] than in MCF/ADR [ （ 18.8±0.4） μg/ g]. Docetaxel caused a concentration-and time-dependent increase in the levels of TP. The TP level in MCF-7 displayed a 1.35-fold increase, while the sensitivity of it displayed a 2.06fold increase. CONCLUSION： Docetaxel causes a concentration- and time-dependent increase in the level of TP.

关 键 词：胸苷磷酸化酶 5-氟尿嘧啶 5’-脱氧-5-氟尿苷 卡培 他滨 多西他赛 

分 类 号：R737.9[医药卫生—肿瘤]