O139群霍乱弧菌β-内酰胺酶相关耐药基因检测及分析  

Study of β-lactamase related resistance genes in Vibrio cholerae O139 serogroup

作　　者：石优章[1] 宋启发[1] 徐景野[1] 金春光[1] 杨元斌[1] 

机构地区：[1]浙江省宁波市疾病预防控制中心,浙江宁波315010

出　　处：《中国卫生检验杂志》2007年第8期1390-1391,1529,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:通过检测分析2004年至2005年21株O139群霍乱弧菌临床分离株对β-内酰胺类抗生素耐药情况和菌株β-内酰胺酶相关耐药基因携带情况,探讨产生耐β-内酰胺类抗生素的分子生物学机制。方法:K-B纸片扩散法药物敏感实验检测细菌耐抗菌药物情况,PCR方法扩增CARB-2、TEM、OXA、VEB和SHV型β-内酰胺酶相关耐药基因,同时用PCR方法检测1型整合子结构。结果:21株O139群霍乱弧菌均对氨苄西林和羧苄西林等耐药,而对头孢噻肟等敏感,PCR均检出TEM-1耐药基因,检出率达100%。未检出CARB-2、OXA、VEB和SHV等基因。测序分析证明该耐药基因与AF397075有高度同源性。PCR定位实验表明该耐药基因不存在于1型整合子内。三相水解实验证明这种耐药为β-内酰胺酶引起。结论:从O139群霍乱弧菌中检出β-内酰胺酶相关TEM-1耐药基因,介导对氨苄西林和羧苄西林等的耐药。Objective：To study the biological molecular mechanism of resistance against β- lactams through testing the resistance patterns and β- lactamase related resistance genes in 21 clinical isolates of Vibrio cholerae O139 serogroup from 2004 to 2005. Methods：Susceptibility toβ-lactam antiobiotics was tested with plate diffusion method. Routine PCR was performed to amplify CARB - 2, VEB, OXA, TEM and SHV genes. Class 1 integron was also detected. Results： Twenty - one were resistant to ampicillin and carboxypenicillin and susceptible to cefotaxime. TEM - 1 resistance genes were found in all isolates through PCR （ 100% ）, which were showed to be highly homologous to AJ634602 （GenBank） through sequencing and did not exist inside the class 1 integron as testified by PCR - mapping. Three - dimensional test was applied to prove that β- lactamase caused this resistance. Conclusion：TEM -1 drug resistance genes are first detected in Vibrio cholerae O139 serogroup which might cause the resistance against ampicillin and carboxypenicillin.

关 键 词：霍乱孤菌 耐药基因 Β-内酰胺酶 

分 类 号：R378.3[医药卫生—病原生物学]