急性肝衰竭大鼠血清TNF-α和IFN-γ/IL-4表达  

Expression of sera TNF-α and IFN-γ/IL-4 in acute hepatic failure rats

作　　者：车德才[1] 李水仙[2] 武延隽[2] 赵中夫[3] 张芸[3] 

机构地区：[1]山西省长治医学院生物学教研室,长治046000 [2]山西省长治医学院微生物学与免疫学教研室 [3]山西省长治医学院肝病研究所

出　　处：《中国公共卫生》2007年第9期1088-1089,共2页Chinese Journal of Public Health

基　　金：山西省自然科学基金(20011073)

摘　　要：目的研究肿瘤坏死因子α(TNF-α)和γ-干扰素/白细胞介素4(IFN-γ/IL-4)在D-氨基半乳糖/内毒素所致大鼠急性肝衰竭中的表达。方法取30只Wistar大鼠,腹腔内注射D-氨基半乳糖/内毒素诱导大鼠急性肝衰竭模型(AHF组);另取30只腹腔内注射等量生理盐水为正常对照组(N)。2组动物于建模后3,6,12,24,48,72 h各取5只动物检测其血清丙氨酸转氨酶(ALT)并观察肝组织的病理形态学变化。酶联免疫吸附试验(ELISA)检测不同时间点动物血清中TNF-α和IFN-γ/IL-4水平的变化。结果结果显示,AHF组肝组织炎性细胞浸润和明显坏死的急性肝衰竭特征,且各时间点血清ALT值均明显高于对照组(P<0.05);AHF组与对照组比较,血清内TNF-α在3 h(P<0.01)、6 h(P<0.05)增高;IFN-γ在3 h(P<0.01)、6 h(P<0.01)增高;IL-4水平在各时间点无明显变化(P>0.05)。结论在D-氨基半乳糖/内毒素所致大鼠急性肝衰竭模型中,TNF-α和IFN-γ对急性肝衰竭早期损伤起重要作用;IL-4可能不参与此种动物模型的病理过程。Objective To research expression of sera TNF-α and IFN-γ/IL-4 in acute hepatic failure models induced by D-glactosamine and endotoxin. Methods Wistar rats with acute hepatic failure（AHF group, n = 30） were injected with saline solution. Sera Alanine Transaminase（ALT） were determined and liver pathological morphology were observed at 3, 6, 12, 24, 48, 72 h following intraperitoneal injection of D-glactosamine and endotoxin, 5 rats per time point; simultaneously, sera TNF-α and IFN-γ/IL-4 were examined by ELISA. Results Infiltration of inflammatory cells and necrosis of hepatic cells were marked in AHF group, ALT increased significantly in AHF group than in N group（ P 〈 0.05） at all time points; Compared with N group, levels of sera tumor necrosis factor-α（TNF-α） in AHF group were obviously higher at 3 h（ P 〈 0.01 ）and 6 h（P 〈 0.05）, and interferon-γ（IFN-γ） were obviously higher at 3 h（ P 〈 0.01 ） and 6 h（ P 〈 0.01 ） ; interleukin-4 （IL-4） weren＇t obviously higher at all time points（ P 〉 0.05 ）. Conclusion TNF-α and IFN-γ play important roles in the AHF models at early phase;However, IL-4 may not contribute to the AHF rats induced by D-glactosamine and endotoxin.

关 键 词：D-氨基半乳糖 内毒素 急笥肝衰竭 肿瘤坏死因子α(TNF-α) γ-干扰素/白细胞介素4(IFN-γ/IL-4) 

分 类 号：R575.3[医药卫生—消化系统]