Fe^(2+),醋酸盐和双氧水对雨生红球藻积累虾青素的影响  

Effect of ferrous ions,acetate and hydrogen peroxide on astaxanthin accumulation in Haematococcus pluvialis

作　　者：高政权[1] 孟春晓[1] 王栋[1] 

机构地区：[1]山东理工大学生命科学院,山东淄博255049

出　　处：《上海水产大学学报》2007年第4期334-340,共7页Journal of Shanghai Fisheries University

基　　金：国家自然科学基金(30671126);山东理工大学科技基金项目资助(4041-306017);山东理工大学博士启动基金项目(4041-405017;4041-405016)

摘　　要：研究了亚铁离子、醋酸盐和双氧水三种化学因子对雨生红球藻积累虾青素的交叉影响和虾青素积累过程中藻体总可溶性蛋白的变化。结果表明,在营养盐缺乏的前提下,添加45 mM醋酸盐和450μM亚铁离子,25℃,24 h连续光照,6 000 lx光强下进行逆境胁迫诱导,能够大大缩短雨生红球藻虾青素积累周期,比空白对照提前一个月使藻细胞完全变红(显微观察)。但诱导过程中会造成部分藻细胞白化,自溶或破壁死亡,虾青素产量比空白对照下降了7.4%;在雨生红球藻虾青素积累过程中,藻体总可溶性蛋白含量逐渐降低,说明雨生红球藻中虾青素的积累可能以蛋白质的消耗为基础。The intersectional effect of NaAC, FeSO4 and H2O2 on the accumulation of astaxanthin in Haematococcus pluvialis wall as well as change of total soluble protein were studied during the process of astaxanthin accumulation of Haematococcus pluvialis . Results showed that in the case of nutrition starvation, 25 ℃ , 6000 lx illumination and additionof NaAC （45 mM） and FeSO4 （450 μM） could advance 30 days for completing astaxanthin accumulation in H. pluvialis cells, but the production of astaxanthin was lower 7.4% than that of blanks. All the treatments with H2O2 had lethal functions to alga cells such as albescent, alga cells thaw and death. SDS-PAGE results displayed the total the protein soluble protein decreased gradually along the process of astaxanthin accumulation in the Haematococcus pluvialis. It is suggested astaxanthin accumulation in Haematococcus pluvialis might be based on protein degradation and depletion.

关 键 词：雨生红球藻 虾青素 总可溶性蛋白 化学因子 

分 类 号：Q949.212[生物学—植物学]