三七三醇皂苷对局灶性脑缺血再灌注大鼠血浆前列环素和血栓素A_2水平的影响  

Effects of PTS on Expression of Prostacyclin and Thromboxane A_2 Following Local Cerebral Ischemia-Reperfusion in Rats

作　　者：陈健文[1] 邱灿华[2] 蓝秀健[2] 马仁强[1] 赖文珊[1] 周小石[1] 刘培庆[1] 

机构地区：[1]中山大学药学院,广东广州510080 [2]中山大学中山医学院,广东广州510080

出　　处：《深圳中西医结合杂志》2007年第4期204-207,共4页Shenzhen Journal of Integrated Traditional Chinese and Western Medicine

基　　金：广东省重点攻关基金(2003C30113);中山大学实验室开放基金项目资助(KF200641)

摘　　要：目的:探讨三七三醇皂苷(PTS)对脑缺血再灌注损伤大鼠血浆中血栓素A2(TXA2)和前列环素(PGI2)水平的影响。方法:将大鼠随机分成6组:假手术组,模型组,PTS25、50、100mg/kg组和阳性对照药血栓通注射液(XST)组;建立局灶性脑缺血再灌注损伤大鼠模型,于造模前分别尾静脉注射给药3d,造模后6h和12h各给药1次,造模24h后取血分离血浆,用放免法测定血浆中TXA2和PGI2的稳定代谢产物TXB2和6-Keto-PGF1α的水平。结果:造模后24h大鼠血浆中TXB2及TXB2/6-Keto-PGF1α(T/P)值明显升高(P<0.01);PGI2则明显降低(P<0.01)。经三七三醇皂苷各剂量预防给药后,大鼠血浆的TXB2含量及TXB2/6-Keto-PGF1α(T/P)值较模型组明显降低(P<0.05或P<0.01),6-Keto-PGF1α含量显著增高(P<0.05或P<0.01),并呈一定的量效依赖关系。结论:三七三醇皂苷能通过降低TXA2合成,升高PGI2,调节TXA2/PGI2比值,达到对大鼠局灶性脑缺血再灌注损伤的保护作用。　 Objective To study the effects of PTS on expression of prostacyclin and thromboxane A2 after cerebral ischemia-reperfusion injury in rats. Methods Sprague dewley rats were divided into 6 groups randomly. They are treated with PTS at the dose of 25 mg/kg, 50 mg/kg, 100 mg/kg, XST 0.45 mL/kg and normal saline injection respectively. The model of acute reperfusion injury after cerebral ischemia in rat was made by middle cerebral artery occlusion. 3 d before MCAO, PTS or normal saline injection or XST were injected via tail vein to the rats respectively. 6 h and 12 h after MCAO, the PTS, XST and saline were given via tail vein to the rats respectively. Blood plasma was taken 24 h later. We observed the effect of PTS on TXB2 and 6-Keto-PGF1α via radioimmunoassay. Results The level of TXB2 and the ratio of TXB2/6-Keto-PGF1α （T/P） in MCAO plasma increased significantly （P 〈 0.01）, while 6-Keto-PGF1α decreased significantly （P 〈 0.01）. PTS could increase the generation of 6-Keto-PGF1α and decrease the level of TXB2 and T/P in MCAO plasma after treated with different dosages. Conclusion PTS has protective effect on ischemia brain injury by increasing the generation of 6-Keto-PGF1α, decreasing the level of TXB2 and improving the balance of TXA2/ PGI2 in blood plasma.

关 键 词：三七三醇皂苷 大脑中动脉闭塞法 脑缺血 血栓素A2 前列环素 

分 类 号：R743.31[医药卫生—神经病学与精神病学]