12个梨品种S基因型的鉴定  

Identification of S-genotypes of Twelve Pear Cultivars by Analysis of DNA Sequence

作　　者：衡伟[1] 张绍铃[1] 张妤艳[1] 吴俊[1] 李秀根[2] 

机构地区：[1]南京农业大学园艺学院,南京210095 [2]中国农业科学院郑州果树研究所,郑州450009

出　　处：《园艺学报》2007年第4期853-858,共6页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(30671437);教育部科技重点项目(105089)

摘　　要：应用PCR扩增技术,根据目的S基因与GenBank中已知S基因同源性100%原理鉴定了我国育成的12个梨品种的S基因型,分别是:‘红酥脆’为S4S36,‘新梨7号’为S28Sd,‘金水3号’为S5S29,‘八月酥’为S3S16,‘金水1号’为S3S29,‘龙泉酥’为S3S22,‘雪花’为S4S16,‘雪芳’为S4S16,‘雅青’为S4S34,‘新雅’为S4S34,‘德胜香’为S3S29,‘富源黄’为S16S33。通过对S基因的DNA序列分析,确认S16、S31为同一S基因。本研究结果丰富了我国梨品种的S基因型信息,为田间合理配置授粉品种提供了依据。Using PCR amplification method, S-genotypes of twelve Chinese pear cuhivars were determined. The S-genotypes were S4S36 for Hongsucui, S28Sd for Xinli 7, S5S29 for Jinshui 3, S3S16 for Bayuesu,S3S29 for Jinshui 1, S3S22 for Longquansu, S4S16 for Xuehua, S4S16 for Xuefang, S4S34 for Yaqing, S4S34 for Xinya, S3S29 for Deshengxiang, S31S33 for Fuyuanhuang. Based on the analysis of the S gene, It was confirmed that S16 and S31 were the same S gene. The study results enriched the genotype information of pear cuhivars in China, providing the basis for appropriate arrangement of pollination tree in pear orchards.

关 键 词：梨 自交不亲和性 DNA序列分析 S基因型 

分 类 号：S661.2[农业科学—果树学]