马铃薯总RNA提取和鉴定方法的改进  

An Improved Method for Extraction and Identification of Potato RNAs

作　　者：白云凤[1] 郭志华[1] 白冬梅[1] 王小琦[1] 张维锋[1] 

机构地区：[1]山西省农业科学院作物遗传研究所,太原030031

出　　处：《园艺学报》2007年第4期1059-1062,共4页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(30471102);山西省回国留学人员资助项目(200689);山西省农业科学院博士基金项目(YBSJJ0604)

摘　　要：对植物总RNA的提取和鉴定方法作了改进。以马铃薯叶片和茎为材料,分别用常规苯酚法和改进法提取总RNA,通过甲醛变性胶和非变性胶及紫外光谱分析,表明用改进法提取的总RNA与常规苯酚法无明显差异,RNA完整性良好,可以用普通琼脂糖凝胶代替甲醛变性胶电泳分析RNA的完整性。RT-PCR结果表明,以改进法提取的总RNA可以用于分子克隆和基因表达分析等分子生物学实验。利用改进法提取植物总RNA,能降低成本,节约时间,也避免了使用DEPC和甲醛等试剂。An improved method of extracting and identifying total RNAs from potato leaf and stem was put forward. The electrophoretic patterns and absorbance at 260 nm and 280 nm in a UV-Vis spectrophotometer revealed that the total RNAs of potato extracted by this method has good completeness and high purity. Furthermore, the resuh of RT - PCR indicated that the RNAs prepared with this improved method could meet the needs of most molecular biological experiment including gene cloning and expression analysis.

关 键 词：马铃薯 总RNA提取 

分 类 号：S653[农业科学—果树学]