人体鼻咽组织的时间分辨自体荧光光谱研究  

Time-resolved Autofluorescence Spectroscopy for Classification of Human Nasopharyngeal Tissues

作　　者：陈明惠[1] 林黎升[1] 杨甫文[2] 李步洪[1] 谢树森[1] 

机构地区：[1]福建师范大学医学光电科学与技术教育部重点实验室,福建福州350007 [2]南京军区福州总医院耳鼻喉科,福建福州350025

出　　处：《激光生物学报》2007年第4期428-431,共4页Acta Laser Biology Sinica

基　　金：卫生部科学研究基金项目(WKJ05-02-012);福建省重大科技建设专项基金(2004YZ03)

摘　　要：实验研究了在397 nm半导体脉冲激光激发下,人体离体鼻咽正常和癌变组织在600 nm荧光发射波长处的时间分辨自体荧光光谱特性。利用双指数衰减方程对时间分辨自体荧光光谱进行拟合后,获得相应的荧光强度随时间的指数衰减方程以及荧光平均寿命。人体鼻咽癌变和正常组织在600 nm处的自体荧光平均寿命分别为(2.94±0.51)ns和(4.29±0.71)ns,两者之间存在显著的差异。应用时间分辨光谱技术的诊断灵敏度和特异性分别为75%和100%。初步表明了时间分辨自体荧光光谱在早期鼻咽癌诊断的应用价值,该方法可望与传统的稳态荧光光谱结合起来,进一步提高早期鼻咽癌荧光诊断的准确率。Time-resolved autofluorescence spectroscopies at 600 nm under 397 nm excitation have been measured for human nasopharyngeal normal and carcinoma tissues in vitro, respectively. The lifetimes of endogenous fluorophores can be indirectly calculated based on the two-component lifetime fitting of time-resolved autofluorescence spectroscopies. Sufficient difference was observed between normal and carcinoma tissues, and the average lifetimes of nasopharyngeal carcinoma and normal tissues are about （2.94±0.51 ） ns and （4.29 ±0.71 ） ns, respectively. In this prospective study, the sensitivity and specificity for diagnosis of nasopharyngeal carcinoma tissue are 75 % and 100 %. Experimental resuits indicate that time-resolved autofluorescence spectroscopy is a very sensitive and promising technique for classification of early nasopharyngeal carcinoma tissue.

关 键 词：时间分辨自体荧光光谱 荧光寿命 鼻咽组织 组织诊断 

分 类 号：Q631[生物学—生物物理学] R739.6[医药卫生—肿瘤]