β-地贫患者红细胞CR1基因组密度多态性的研究  

Investigation on polimorphism of CR1 gene density of erithrocyte in patients with beta-Thalassemia

作　　者：覃志坚[1] 何印蕾[1] 郑子敏[1] 农乐根[1] 常正义[1] 

机构地区：[1]右江民族医学院附属医院检验科,广西百色533000

出　　处：《中国现代医学杂志》2007年第16期1938-1940,共3页China Journal of Modern Medicine

基　　金：广西自然科学基金资助项目(0135034)

摘　　要：目的探讨β-地中海贫血患者红细胞CR1基因组密度多态性分布及CR1数量的变化。方法采用PCR和HindⅢ酶切技术(RFLP)和酵母菌花环试验法分别测定β-地中海贫血患者红细胞CR1基因组密度多态性和CR1的数量,并与健康对照组作比较。结果β-地中海贫血患者HH型占40.91%(45/110),HL型占27.27%(30/110),LL型占31.82%(35/110);健康对照组HH型占55%(55/100),HL型占40%(40/100),LL型占5%(5/100);β-地中海贫血患者RBC-CRIR为(10.86±1.15),健康对照组(26.72±1.29);β-地中海贫血患者RBC-ICR为(8.63±0.32),健康对照组(3.91±0.13)。其中HH型和HL型与健康对照组比较差异无显著性(P>0.05),LL型与健康对照组比较差异有显著性(P<0.01)。结论β-地中海贫血患者CR1基因LL型比率高且红细胞免疫黏附功能降低,提示可能与β-地中海贫血的发病率相关。[Objective] To study CR1 density polymorphism and the RBC C3b receptor （ECR1） on erythrocytes of patients with β_The mediterranean sea anaemia to distribute suffer of cuutain to measure the variety. [Method] Polymorphism chain reaction （PCR） was used to amplify the genome DNA fragment of in an intron of the CR1 structural gene, followed gy Hind Ⅲ endomuclease digestion, and measurese the RBC repair to suffer the C3b receptor the quantity measurement, and same time, combined to make the normal matched control comparison. [Result] The β mediterranean sea anaemia of HH was 40.91% （45/110）, HL was 27.27% （30/110）, and LL was 31.82% （35/110）; the comparison of the healthes HH was 55% （55/100）, HL was 40% （40/100）, LL was 5% （5/100）; the β-The mediterranean sea anaemia of RBC-CRIR was （10.86±1.15） and normal comparison was （26.72±1.29）; the β-The mediterranean sea anaemia of RBC-ICR was （8.63±0.32） and normal comparison was （3.91±0.13）. So ECR1 the quantity CR1 activated of LL ware more obvionsly lower than matched control （P 〈0.01） liquid immunityinty index sign all contain different difference. [Conclusion] The gene CR1-LL of β-The mediterranean sea anaemias ware high rate and erythrocytes of the immunity glue to attach the function lower than normal comparison.

关 键 词：Β-地中海贫血 红细胞CR1基因 红细胞免疫黏附功能 

分 类 号：Q343[生物学—遗传学]