人乳腺珠蛋白信使RNA聚合酶联反应检测乳腺癌前哨淋巴结微转移  

Detection of breast cancer micrometastase sentinel lymph node by using hMAM mRNA RT-PCR

作　　者：张良[1] 僧靖静[2] 杨振[2] 

机构地区：[1]河南省安阳市第六人民医院普外科,河南安阳455000 [2]郑州大学第一附属医院普外科,河南郑州450052

出　　处：《中国现代医学杂志》2007年第16期1950-1953,共4页China Journal of Modern Medicine

基　　金：河南省高校创新人才培养对象基金资助项目资助HN2005-2010

摘　　要：目的应用RT-PCR法检测乳腺癌前哨淋巴结(sentinel lymph node,SLN)中乳腺珠蛋白基因(human mammaglobin,hMAM)mRNA的表达情况。探讨乳腺癌SLN定位及其微转移检测的临床意义。方法对51例乳腺癌患者采用术中肿瘤周围和乳晕下注射专利蓝的方法定位SLN,检测SLN中hMAM mR-NA的表达情况。结果SLN定位成功率为76.47%(39/51);RT-PCR检测hMAM mRNA与常规病理检查方法相比,检测SLN转移的阳性率提高了20.51%,二者差异具有显著性(P<0.05)。结论hMAM mR-NA可作为标志物来检测乳腺癌SLN中的微转移情况,通过SLN定位和RT-PCR的联合应用,可明显提高乳腺癌SLN微转移的检出率,对预测腋窝淋巴结(axillary lymph node,ALN)的状态有着重要的临床价值。[Objective] To detect the breast cancer micrometastases in sentinel lymph node （SLN） by using hMAM mRNA RT-PCR and to evaluate the clinical significance. [Methods] In fifty-one breast cancer patients, patten blue-V dye was injected into multiple sites around the tumor and subareolars to locate the SLN, and RT-PCR was used to examine the expressions of hMAM mRNA. [Results] The SLN was successfully identified in 76.47% （39/51） of the patients. Detection rate of SLN metastases increased 20.51% by applying PT-PCR methods. There was a significant difference between RT-PCR methods and routine pathological examination （P 〈0.05）. [Conclusion] hMAM mRNA may be potential tumor marker for detecting micrometastasis SLN of breast cancer. The combined utilization of locating SLN and RT-PCR can greatly enhance the efficiency of micrometastases of the SLN in breast cancer.

关 键 词：乳腺肿瘤 RT-PCR hMAMmRNA 微转移 

分 类 号：R737.9[医药卫生—肿瘤]