RP-HPLC法测定茶叶中绿原酸和没食子酸含量的研究  

Determination of Chlorogenic Acid and Gallic Acid in Tea by RP-HPLC

作　　者：黄宏伟[1] 邹佑云[2] 邹盛勤[3] 

机构地区：[1]宜春学院化学与生物工程学院,江西宜春336000 [2]宜春学院美容医学院,江西宜春336000 [3]江西省天然药物活性成分研究重点实验室宜春学院生物工程研究所,江西宜春336000

出　　处：《安徽农业科学》2007年第25期7752-7753,共2页Journal of Anhui Agricultural Sciences

摘　　要：在建立反相高效液相色谱法的同时,测定不同产地茶叶中没食子酸和绿原酸的含量。结果表明：当绿原酸进样量0.092～0.92μg,没食子酸进样量0.075 6～0.756 0μg时,回归方程具有良好的线性关系;该方法准确、快速、重现性好,可用于茶叶中没食子酸和绿原酸含量的测定;5个不同产地的茶叶中没食子酸和绿原酸含量有明显差异。A RP-HPLC method for determination of chlorogenic acid and gallic acid in tea from different regions was established.The results showed that the liner range of chlorogenic acid was 0.092～0.92 μg,and the liner range of gallic acid was 0.075 6～0.756 0 μg.The method was accurate,rapid,reproducible and suitable for determination of chlorogenic acid and gallic acid in tea. There were significant differences in the contents of chlorogenic acid and gallic acid in tea from five regions.

关 键 词：茶叶 绿原酸 没食子酸 高效液相色谱 光电二极管阵列检测器 

分 类 号：O657.72[理学—分析化学]