拟南芥Ran2小GTP结合蛋白抗血清的制备及其FITC荧光标记  

Preparation and Label with FITC of Antibody against Arabidopsis thaliana Ran2 Small GTP-Binding Protein

作　　者：马立安[1] 江涛[2] 张忠明[3] 

机构地区：[1]长江大学生命科学学院,荆州434025 [2]长江大学动物科学学院,荆州434025 [3]华中农业大学农业微生物学国家重点实验室,武汉430070

出　　处：《华中农业大学学报》2007年第4期439-442,共4页Journal of Huazhong Agricultural University

基　　金：国家自然科学基金项目(30070370);美国国家科学基金项目(NSF-MCB0548525)资助

摘　　要：将原核表达、纯化的Ran2蛋白对新西兰兔进行免疫制备抗血清,ELISA测定其效价为1∶51 200。免疫印迹分析表明,原核表达、纯化的Ran2和拟南芥可溶性蛋白杂交带均在26 ku处。FITC荧光标记抗体检测Ran2绿色杂交带主要出现在细胞分裂间期的核膜上。The purified Ran2 protein from E. coli was used to raise antibodies in rabbits. The result of ELISA showed that the titer of Ran2 antibody was 1 ： 51 200. Western blot assay revealed that the Ran2 antibody reacted with a 26 ku polypeptide band from the recombinant Ran2 purified from E. coli and the soluble protein of Arabidopsis seedlings. The result of Ran2 antibody labeled with fluorescein isothiocyanate （FITC） staining 3-day old onion root tip cells displayed that green band was mainly localized to the nuclear envelope in interphase.

关 键 词：Ran2小GTP结合蛋白 抗血清 免疫印迹 FITC荧光标记 

分 类 号：Q503[生物学—生物化学]