温度对南极红酵母Rhodotorula sp. NJ298抗氧化系统及质膜流动性的影响  

Effect of temperature on antioxidant system and plasma membrane fluidity of Antarctic yeast Rhodotorula sp. NJ298

作　　者：刘均玲[1] 黄勃[1] 李江[2] 李光友[2] 缪锦来[2] 

机构地区：[1]海南大学海洋学院,海南海口570228 [2]国家海洋局海洋生物活性物质重点实验室,山东青岛266061

出　　处：《中国海洋药物》2007年第4期21-26,共6页Chinese Journal of Marine Drugs

基　　金：国家自然科学基金资助项目(No.40406003)

摘　　要：目的研究在不同温度下南极红酵母Rhodotorula sp. NJ298抗氧化酶活性变化,丙二醛(MDA)、类胡萝卜素含量变化,及细胞质膜流动性的变化。方法采用分光光度法研究了-3,0,8℃3种温度下培养的南极红酵母Rhodotorula sp. NJ298超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)活性,及丙二醛(MDA)和类胡萝卜素含量,同时测定了对H2O2的抗性,并应用荧光探针法测定了质膜流动性。结果在-3℃低温时,NJ298 SOD酶活性下降,CAT和POD酶活性升高,MDA含量没有明显变化,类胡萝卜素含量增加,对H2O2的抗性增加,质膜维持正常流动性。结论在低温时,NJ298类胡萝卜素积累,在积累过程中,虽然SOD酶活力下降,但类胡萝卜素替代SOD酶行使主要的抗氧化功能,并使NJ298抗氧化能力增强,保护膜系统,维持质膜正常流动性,这在NJ298低温适应性中具有重要作用。Objective To investigate the effect of temperature on the activity of antioxidant enzyme, the content of MDA and carotenoids, and the plasma membrane fluidity of Antarctic yeast Rhodotorula sp. NJ298. Methods The activity of antioxidant enzyme, the contents of MDA and carotenoids were stuidied by using spectrophotometer method, as well as the fluidity of plasma membrane was determined by measuring fluorescence polarization in Rhodotorula sp. NJ298 treated with different temperatures, -3, 0, 8 ℃. Results The results were as follows： at -3 ℃ the activity of SOD decreased, and the activities of CAT and POD increased, the content of carotenoids increased obviously, the content of MDA had no change obviously, and the fluidity of plasma membrane kept stable at different temperatures. Conclusion These results indicated at -3 ℃ Rhodotorula sp. NJ298 carotenoids played an antioxidant role which substituted for SOD, increased the antioxidant ability of Rhodotorula sp. NJ298, and maintained an optimum membrane fluidity. In a word, the antioxidant system played an important role in the adaptation of Antarctic sea ice microorganisms in low temperature.

关 键 词：南极酵母Rhodotorula sp.NJ298 抗氧化 质膜流动性 低温适应 

分 类 号：R931.711[医药卫生—生药学] R965[医药卫生—药学]