金黄色葡萄球菌氟喹诺酮的耐药性与grlA、grlB突变的关系  

Relation between fluoroquinolones-resistant and mutation of grlA and grlB gene in Staphylococcus aureus

作　　者：杜锐[1] 韩文瑜[1] 雷连成[1] 

机构地区：[1]吉林大学农学部畜牧兽医学院

出　　处：《西北农林科技大学学报（自然科学版）》2007年第9期5-9,共5页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家自然科学基金项目(30170698);吉林农业大学博士启动基金资助项目

摘　　要：为了探讨金黄色葡萄球菌的氟喹诺酮耐药性与grlA、grlB基因突变的关系,从52株野生型金黄色葡萄球菌中筛选出1株对氟喹诺酮敏感的金黄色葡萄球菌,对其进行耐药诱导,获得一系列不同氟喹诺酮耐药水平的金黄色葡萄球菌,并对其grlA、grlB基因进行PCR扩增、测序及序列分析。结果表明,金黄色葡萄球菌的氟喹诺酮耐药性与grlB基因突变无关,而与grlA的丝氨酸(Ser)80→苯丙氨酸(Phe)和谷氨酸(Glu)84→赖氨酸(Lys)突变密切相关。金黄色葡萄球菌grlA的80位氨基酸和84位氨基酸双突变是其氟喹诺酮耐药性提高的标志之一。The purpose was to study the relation between fluoroquinolones-resistant and mutation of grlA and grlB gene in staphylococcus, aureus,the sensitive strain was induced with Ciprofloxacin to get grad-resistance strain of S, aureus, the grlA and grlB gene were amplified and sequenced by polymerase chain reaction. Sequence analysis showed that grlB gene had no mutation,while Ser 80→Phe and Glu 84→ Lys of the grlA gene happened. These assays demonstrated that Ser 80→Phe and Glu 84→Lys of the grlA mutation were the main mark of staphylococcus aureus to {luoroquinolones-resistant rising.

关 键 词：金黄色葡萄球菌 氟喹诺酮 耐药性 grlA grlB 基因突变 

分 类 号：Q936[生物学—微生物学] R978.11[医药卫生—药品]