我国主要养殖罗非鱼的16S rRNA序列特征分析  

Sequence analysis of mitochondrial 16S rRNA in Oreochromis species in China

作　　者：郭奕惠[1] 黄桂菊[1] 喻达辉[1] 李莉好[1] 符云 叶卫 

机构地区：[1]中国水产科学研究院南海水产研究所,广东广州510300 [2]国家级广东罗非鱼良种场,广东广州511453

出　　处：《上海水产大学学报》2007年第5期490-494,共5页Journal of Shanghai Fisheries University

基　　金：广州番禺区科技计划项目(2004-Z-61-1)

摘　　要：对我国主要养殖的尼罗、奥利亚、莫桑比克、杂交种尼奥(尼罗♀×奥利亚♂)和红罗非鱼(莫桑比克×尼罗)的线粒体16S rRNA部分序列进行了PCR扩增和测序分析,探讨罗非鱼种间亲缘关系和种类鉴别标记。PCR产物经直接测序,5种罗非鱼中大部分个体均获得长546 bp的基因序列。其碱基组成GC含量为47.4%。序列比对分析显示变异位点18个,没有插入/缺失位点,转换/颠换比率为2.7。序列分析表明,红和奥利亚各有2个单倍型,尼奥、莫桑比克与尼罗各有1个单倍型。其中,尼奥与尼罗的单倍型序列相同,莫桑比克与红罗非鱼的1个单倍型序列相同。表明部分红罗非鱼的母本为莫桑比克。UPGMA聚类分析表明,尼奥与尼罗聚成一支、红罗非鱼与莫桑比克成一支、奥利亚独成一支。Mitochondrial 16S rRNA gene sequence variation of Nile tilapia （Oreochromis niloticus）, blue tilapia （ O. aureus）, Mozambique tilapia （ O. mossambicus）, hybrid tilapia （ O. niloticus 9 × O. aureus ♂ ） and red tilapia （O. mossambicus × O. niloticus ） was investigated-in order to understand the genetic relationship among them as well as to provide interspecific molecular markers for breeding practice. The partial sequence of 16S rRNA was 546 bp long for the five species as revealed by directly sequencing the PCR. products. GC content was 47.4%. Alignment revealed 18 variable sites including 15 parsimony - informative sites and 3 singletons but no insertion / deletions with transition / transversion ratio of 2.7. Sequence analysis indicated that there are five haplotypes ： haplotype 1 and 2 （ blue tilapias）, haplotype 3 （ the hybrid and Nile tilapias）, haplotype 4 （the red and Mozambique tilanias） and haplotype 5 （ red tilapia）. Accordingly the five species can be divided into three groups. It also suggests that O. mossambicus is the female parent of some red tilapias. Phylogenetic analysis suggested that the group of O. niloticus and the hybrid O. niloticus × O. aureus are closely related with the group of O. mossambicus and the red tilapia, whereas O. aureus is genetically distinct from the other four species.

关 键 词：罗非鱼 16SRRNA基因 种类鉴定 序列特征 

分 类 号：S917[农业科学—水产科学]