铜、镉对鮸鱼幼鱼鳃丝Na^+-K^+-ATPase和肝脏SOD酶活性的影响  

Effects of Cu^(2+) and Cd^(2+) on Na^+-K^+-ATPase activity of gills and superoxide dismutase activity of liver in Miichthys miiuy

作　　者：柳敏海 罗海忠 陈波 彭志兰 傅荣兵 

机构地区：[1]浙江省舟山市水产研究所,浙江舟山316000

出　　处：《安全与环境学报》2007年第4期5-8,共4页Journal of Safety and Environment

基　　金：浙江省重大农业科技攻关项目(2004C12028)

摘　　要：以鮸鱼(Miichthysmiiuy)幼鱼为研究对象,研究了Cu2+和Cd2+对鮸鱼幼鱼鳃丝Na+-K+-ATPase和肝脏SOD酶活性的影响。以6个Cu2+浓度(0.01mg/L、0.05mg/L、0.1mg/L、0.2mg/L、0.4mg/L和0.8mg/L)和6个Cd2+浓度(0.005mg/L、0.025mg/L、0.05mg/L、0.1mg/L、0.2mg/L、0.4mg/L)对鮸鱼幼鱼进行8d染毒。结果表明,Cu2+和Cd2+各处理组鱼鳃丝Na+-K+-ATPase活力随取样时间的变化显著(P<0.05),且呈峰值变化,在1d时达到最大值,然后缓慢下降,最后各处理组酶活力趋于稳定。两种重金属离子对鮸鱼鳃丝Na+-K+-ATPase活力的影响在同一取样时间各处理组间的差异也显著(P<0.05),其影响程度与重金属的浓度呈负相关,且Cd2+浓度为0.4mg/L时,在第8天酶活力与对照组的差异不显著(P>0.05)。Cu2+和Cd2+在1～8d对鮸鱼鳃丝Na+-K+-ATPase活力的诱导率表现为Cu2+>Cd2+。低浓度组Cu2+和Cd2+曝露时,鮸鱼肝组织中SOD活性变化在短时间内不明显(P>0.05),但随着时间的延长,SOD活性提高,与对照组差异显著(P<0.05),导致"毒物兴奋效应"。高浓度Cu2+和Cd2+曝露时,随着时间的延长和浓度的增强,肝组织SOD的活力抑制越明显(P<0.05)。The present paper intends to introduce the experimental results of ours on the effects of Cu^2＋ and Cd^2＋ on Na^＋ - K^＋ - ATPase activity, of gills and superoxid dismutase activity of the liver in Miichthys miiuy. In our experiments, we have adopted early cultivated juvenile Miichthys miiuy with their average weight between 10.0 - 11.2 g and average length of about 9.5 - 10.0 cm. As a rule, it took usually 8 days to domesticate the fish before the experiments began. The results of our experhnents indicate that Cu^2＋ （0.01 mg/L, 0.05 mg/L, 0.1 mg/L, 0.2 mg/L, 0.4 mg/L and 0.8 mg/L） and Cd^2＋ （0.005 mg/L, 0.025 mg/L, 0.05 mg/L, 0.1 mg/L, 0.2 mg/L, 0.4 mg/L） may produce significant effect （P 〈 0.05） on the Na^＋ - K^＋ - ATPase activities with the changing curve of the Na^＋ - K^＋ - AT- Pase activity reaching its marked peak value on the first day. At the same time, the Na^＋- K^＋ ATPase activity in each group of the two kinds of metal ions also proved to be significant （P 〈 0.05）, though the relation of the activity with their concentration seems negative in nature. Meanwhile, the inductive ration of the two heavy metal ions on the Na^＋ - K^＋ - ATPase activities prove to be Cu^2 ＋ 〉 Cd^2 ＋ . Thus, the results of our experiments indicate that the activities of SOD in their liver have not brought any obvious changes at low Cu^2＋ and Cd^2＋ concentrations in a short time. However, the activities can be promoted with prolonging the exposure time, which can probably be expected to come about as a result of the so-called ＂toxic excitement effect＂.

关 键 词：环境毒理学 鮸鱼 CU^2+ CD^2+ NA^+-K^+-ATPASE SOD 

分 类 号：X503.225[环境科学与工程—环境工程]